Seroatlas · Human Serome Atlas

USP44

Ubiquitin carboxyl-terminal hydrolase 44

Also known as: FLJ14528, UBP44_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H0E7
Gene: USP44
Ensembl: ENSG00000136014
Chromosome: 12
Canonical length: 712 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoli fibrillar center,Microtubules

OverviewNCBI Gene

The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset. This protein specifically deubiquitinates CDC20, which stabilizes the anaphase promoting complex/cyclosome. [provided by RefSeq, Dec 2016]

Canonical amino-acid sequenceUniProt

712 residues, UniProt reviewed canonical sequence.

>Q9H0E7|USP44
     1  MLAMDTCKHV GQLQLAQDHS SLNPQKWHCV DCNTTESIWA CLSCSHVACG RYIEEHALKH
    61  FQESSHPVAL EVNEMYVFCY LCDDYVLNDN TTGDLKLLRR TLSAIKSQNY HCTTRSGRFL
   121  RSMGTGDDSY FLHDGAQSLL QSEDQLYTAL WHRRRILMGK IFRTWFEQSP IGRKKQEEPF
   181  QEKIVVKREV KKRRQELEYQ VKAELESMPP RKSLRLQGLA QSTIIEIVSV QVPAQTPASP
   241  AKDKVLSTSE NEISQKVSDS SVKRRPIVTP GVTGLRNLGN TCYMNSVLQV LSHLLIFRQC
   301  FLKLDLNQWL AMTASEKTRS CKHPPVTDTV VYQMNECQEK DTGFVCSRQS SLSSGLSGGA
   361  SKGRKMELIQ PKEPTSQYIS LCHELHTLFQ VMWSGKWALV SPFAMLHSVW RLIPAFRGYA
   421  QQDAQEFLCE LLDKIQRELE TTGTSLPALI PTSQRKLIKQ VLNVVNNIFH GQLLSQVTCL
   481  ACDNKSNTIE PFWDLSLEFP ERYQCSGKDI ASQPCLVTEM LAKFTETEAL EGKIYVCDQC
   541  NSKRRRFSSK PVVLTEAQKQ LMICHLPQVL RLHLKRFRWS GRNNREKIGV HVGFEEILNM
   601  EPYCCRETLK SLRPECFIYD LSAVVMHHGK GFGSGHYTAY CYNSEGGFWV HCNDSKLSMC
   661  TMDEVCKAQA YILFYTQRVT ENGHSKLLPP ELLLGSQHPN EDADTSSNEI LS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against USP44 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.38
Highest tissue expression: 23 nTPM

Expression across tissuesHPA

Tissue

testis: 23 nTPM
cerebellum: 3.7 nTPM
lung: 3.6 nTPM
thymus: 3.4 nTPM
pancreas: 2.2 nTPM
parathyroid gland: 1.6 nTPM

Single-cell type

early spermatids: 481 nCPM
late primary spermatocytes: 448 nCPM
late spermatids: 131 nCPM
alveolar cells type 2: 43 nCPM
pituicytes/fscs: 41 nCPM
transitional alveolar cells: 41 nCPM

Immune cell

naive CD4 T-cell: 6.1 nTPM
memory B-cell: 4.7 nTPM
naive CD8 T-cell: 4.1 nTPM
naive B-cell: 3.8 nTPM
MAIT T-cell: 2.4 nTPM
memory CD4 T-cell: 1.7 nTPM

Brain region

cerebellum: 15 nTPM
white matter: 2.8 nTPM
pons: 2.5 nTPM
cerebral cortex: 2.4 nTPM
midbrain: 2.2 nTPM
basal ganglia: 2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.07
gnomAD pLI: 0
gnomAD missense Z: 0.65
DepMap mean gene effect: -0.17
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of USP44 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads USP44 as an antibody target. Whether an autoantibody or antibody against USP44 could matter depends on whether native USP44 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

USP44 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label USP44 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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