USF1

Protein identityUniProt · HPA

UniProt accession

P22415

Gene

USF1

Ensembl

ENSG00000158773

Chromosome

1

Canonical length

310 aa

Protein class

Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21. [provided by RefSeq, Feb 2013]

Canonical amino-acid sequenceUniProt

310 residues, UniProt reviewed canonical sequence.

>P22415|USF1
     1  MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV FRTENGGQVM
    61  YRVIQVSEGQ LDGQTEGTGA ISGYPATQSM TQAVIQGAFT SDDAVDTEGT AAETHYTYFP
   121  STAVGDGAGG TTSGSTAAVV TTQGSEALLG QATPPGTGQF FVMMSPQEVL QGGSQRSIAP
   181  RTHPYSPKSE APRTTRDEKR RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK
   241  GGILSKACDY IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG
   301  LEVVIKNDSN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against USF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.61

Highest tissue expression

34 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about USF1.

Disease | AllUniProt

Conditions USF1 is implicated in, by any mechanism.

• Hyperlipidemia, familial combined, 1 (FCHL1) MIM:602491

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.59

gnomAD pLI

0.02

gnomAD missense Z

2.14

DepMap mean gene effect

-0.17

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to glucose stimulus
• cellular response to insulin stimulus
• glucose homeostasis
• glucose mediated signaling pathway
• glucose metabolic process
• late viral transcription
• lipid homeostasis
• negative regulation of fibrinolysis
• positive regulation of transcription by RNA polymerase II
• positive regulation of transcription from RNA polymerase II promoter by glucose
• regulation of transcription by RNA polymerase II
• response to hypoxia
• response to UV
• carbon catabolite regulation of transcription

Molecular functions

• bHLH transcription factor binding
• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity, RNA polymerase II-specific
• E-box binding
• enzyme binding
• histone deacetylase binding
• identical protein binding
• protein heterodimerization activity
• protein homodimerization activity
• protein kinase binding
• protein-containing complex binding
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• chromatin
• Golgi apparatus
• nucleoplasm
• nucleus
• transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• Myc-type, basic helix-loop-helix (bHLH) domain
• Helix-loop-helix DNA-binding domain superfamily
• Upstream Stimulatory Factor
• Helix-loop-helix DNA-binding domain

KeywordsUniProt

• DNA-binding
• Isopeptide bond
• Nucleus
• Transcription
• Transcription regulation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of USF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• FOSL1
• USF2