USF2
Upstream stimulatory factor 2
Also known as: bHLHb12, FIP, USF2_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q15853
- Gene
- USF2
- Ensembl
- ENSG00000105698
- Chromosome
- 19
- Canonical length
- 346 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm,Vesicles
- Quaternary structure
- Homodimer
OverviewNCBI Gene
This gene encodes a member of the basic helix-loop-helix leucine zipper family of transcription factors. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs and is involved in regulating multiple cellular processes. [provided by RefSeq, Mar 2016]
Canonical amino-acid sequenceUniProt
346 residues, UniProt reviewed canonical sequence.
>Q15853|USF2
1 MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG
61 DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS VVSTAAFAGG QQAVTQVGVD
121 GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF SNGGSPAAEA VSGEARFAYF PASSVGDTTA
181 VSVQTTDQSL QAGGQFYVMM TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH
241 NEVERRRRDK INNWIVQLSK IIPDCNADNS KTGASKGGIL SKACDYIREL RQTNQRMQET
301 FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GEGTRQLocalizationUniProt · AlphaFold · HPA
Whether an antibody against USF2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.63
- Highest tissue expression
- 337 nTPM
Expression across tissuesHPA
Tissue
- skeletal muscle: 337 nTPM
- basal ganglia: 219 nTPM
- amygdala: 203 nTPM
- cerebral cortex: 199 nTPM
- cerebellum: 198 nTPM
- spinal cord: 198 nTPM
Single-cell type
- platelets: 401 nCPM
- pdcs: 143 nCPM
- retinal pigment epithelial cells: 102 nCPM
- neutrophils: 97 nCPM
- esophageal apical cells: 88 nCPM
- monocytes: 85 nCPM
Immune cell
- plasmacytoid DC: 51 nTPM
- basophil: 45 nTPM
- T-reg: 14 nTPM
- naive B-cell: 14 nTPM
- myeloid DC: 14 nTPM
- NK-cell: 12 nTPM
Brain region
- medulla oblongata: 188 nTPM
- thalamus: 186 nTPM
- cerebral cortex: 186 nTPM
- basal ganglia: 183 nTPM
- cerebellum: 183 nTPM
- amygdala: 178 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.25
- gnomAD pLI
- 0.99
- gnomAD missense Z
- 1.89
- DepMap mean gene effect
- -0.12
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cellular response to glucose stimulus
- glucose mediated signaling pathway
- lactation
- late viral transcription
- lipid homeostasis
- positive regulation of transcription by RNA polymerase II
- positive regulation of transcription from RNA polymerase II promoter by glucose
- regulation of transcription by RNA polymerase II
Molecular functions
- bHLH transcription factor binding
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity
- DNA-binding transcription factor activity, RNA polymerase II-specific
- E-box binding
- protein heterodimerization activity
- protein homodimerization activity
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- sequence-specific double-stranded DNA binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of USF2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads USF2 as an antibody target. Whether an autoantibody or antibody against USF2 could matter depends on whether native USF2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
USF2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label USF2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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