Seroatlas · Human Serome Atlas

UBE4B

Ubiquitin conjugation factor E4 B

Also known as: E4, KIAA0684, UBE4B_HUMAN, UBOX3, UFD2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O95155
Gene: UBE4B
Ensembl: ENSG00000130939
Chromosome: 1
Canonical length: 1302 aa
Protein class: Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1302 residues, UniProt reviewed canonical sequence.

>O95155|UBE4B
     1  MEELSADEIR RRRLARLAGG QTSQPTTPLT SPQRENPPGP PIAASAPGPS QSLGLNVHNM
    61  TPATSPIGAS GVAHRSQSSE GVSSLSSSPS NSLETQSQSL SRSQSMDIDG VSCEKSMSQV
   121  DVDSGIENME VDENDRREKR SLSDKEPSSG PEVSEEQALQ LVCKIFRVSW KDRDRDVIFL
   181  SSLSAQFKQN PKEVFSDFKD LIGQILMEVL MMSTQTRDEN PFASLTATSQ PIAAAARSPD
   241  RNLLLNTGSN PGTSPMFCSV ASFGASSLSS LYESSPAPTP SFWSSVPVMG PSLASPSRAA
   301  SQLAVPSTPL SPHSAASGTA AGSQPSSPRY RPYTVTHPWA SSGVSILSSS PSPPALASSP
   361  QAVPASSSRQ RPSSTGPPLP PASPSATSRR PSSLRISPSL GASGGASNWD SYSDHFTIET
   421  CKETDMLNYL IECFDRVGIE EKKAPKMCSQ PAVSQLLSNI RSQCISHTAL VLQGSLTQPR
   481  SLQQPSFLVP YMLCRNLPYG FIQELVRTTH QDEEVFKQIF IPILQGLALA AKECSLDSDY
   541  FKYPLMALGE LCETKFGKTH PVCNLVASLR LWLPKSLSPG CGRELQRLSY LGAFFSFSVF
   601  AEDDVKVVEK YFSGPAITLE NTRVVSQSLQ HYLELGRQEL FKILHSILLN GETREAALSY
   661  MAAVVNANMK KAQMQTDDRL VSTDGFMLNF LWVLQQLSTK IKLETVDPTY IFHPRCRITL
   721  PNDETRVNAT MEDVNDWLTE LYGDQPPFSE PKFPTECFFL TLHAHHLSIL PSCRRYIRRL
   781  RAIRELNRTV EDLKNNESQW KDSPLATRHR EMLKRCKTQL KKLVRCKACA DAGLLDESFL
   841  RRCLNFYGLL IQLLLRILDP AYPDITLPLN SDVPKVFAAL PEFYVEDVAE FLFFIVQYSP
   901  QALYEPCTQD IVMFLVVMLC NQNYIRNPYL VAKLVEVMFM TNPAVQPRTQ KFFEMIENHP
   961  LSTKLLVPSL MKFYTDVEHT GATSEFYDKF TIRYHISTIF KSLWQNIAHH GTFMEEFNSG
  1021  KQFVRYINML INDTTFLLDE SLESLKRIHE VQEEMKNKEQ WDQLPRDQQQ ARQSQLAQDE
  1081  RVSRSYLALA TETVDMFHIL TKQVQKPFLR PELGPRLAAM LNFNLQQLCG PKCRDLKVEN
  1141  PEKYGFEPKK LLDQLTDIYL QLDCARFAKA IADDQRSYSK ELFEEVISKM RKAGIKSTIA
  1201  IEKFKLLAEK VEEIVAKNAR AEIDYSDAPD EFRDPLMDTL MTDPVRLPSG TIMDRSIILR
  1261  HLLNSPTDPF NRQTLTESML EPVPELKEQI QAWMREKQNS DH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against UBE4B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.36
Highest tissue expression: 34 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 34 nTPM
tongue: 26 nTPM
skin: 24 nTPM
pancreas: 22 nTPM
cerebellum: 21 nTPM
esophagus: 20 nTPM

Single-cell type

neutrophils: 372 nCPM
retinal ganglion cells: 371 nCPM
adrenal cortex cells: 343 nCPM
myonuclei: 301 nCPM
oligodendrocytes: 283 nCPM
rod photoreceptor cells: 238 nCPM

Immune cell

T-reg: 6.4 nTPM
neutrophil: 5.5 nTPM
MAIT T-cell: 5.1 nTPM
memory CD4 T-cell: 5 nTPM
memory CD8 T-cell: 5 nTPM
classical monocyte: 4.9 nTPM

Brain region

white matter: 79 nTPM
pons: 65 nTPM
cerebellum: 62 nTPM
basal ganglia: 59 nTPM
medulla oblongata: 57 nTPM
thalamus: 57 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.16
gnomAD pLI: 1
gnomAD missense Z: 3.73
DepMap mean gene effect: -0.27
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

ERAD pathway
neuron projection development
proteasome-mediated ubiquitin-dependent protein catabolic process
protein autoubiquitination
protein monoubiquitination
protein polyubiquitination
response to UV
ubiquitin-dependent protein catabolic process
ventricular trabecula myocardium morphogenesis
granzyme-mediated apoptotic signaling pathway

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of UBE4B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads UBE4B as an antibody target. Whether an autoantibody or antibody against UBE4B could matter depends on whether native UBE4B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

UBE4B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label UBE4B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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