Seroatlas · Human Serome Atlas

U2AF1L4

Splicing factor U2AF 26 kDa subunit

Also known as: MGC33901, U2AF1L3, U2af26, U2AF4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8WU68
Gene: U2AF1L4
Ensembl: ENSG00000161265
Chromosome: 19
Canonical length: 220 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable pre-mRNA 3'-splice site binding activity. Predicted to be involved in mRNA splicing, via spliceosome. Predicted to be located in nucleoplasm. Predicted to be part of U2AF complex and spliceosomal complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

220 residues, UniProt reviewed canonical sequence.

>Q8WU68|U2AF1L4
     1  MAEYLASIFG TEKDKVNCSF YFKIGVCRHG DRCSRLHNKP TFSQTIVLLN LYRNPQNTAQ
    61  TADGSHCHVS DVEVQEHYDS FFEEVFTELQ EKYGEIEEMN VCDNLGDHLV GNVYVKFRRE
   121  EDGERAVAEL SNRWFNGQAV HGELSPVTDF RESCCRQYEM GECTRGGFCN FMHLRPISQN
   181  LQRQLYGRGP RRRSPPRFHT GHHPRERNHR CSPDHWHGRF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against U2AF1L4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.37
Highest tissue expression: 12 nTPM

Expression across tissuesHPA

Tissue

basal ganglia: 12 nTPM
heart muscle: 11 nTPM
cerebral cortex: 10 nTPM
amygdala: 9.6 nTPM
choroid plexus: 9.5 nTPM
hippocampal formation: 9 nTPM

Single-cell type

late primary spermatocytes: 32 nCPM
tuft cells: 23 nCPM
plasma cells: 22 nCPM
late spermatids: 21 nCPM
early primary spermatocytes: 20 nCPM
syncytiotrophoblasts: 18 nCPM

Immune cell

T-reg: 10 nTPM
plasmacytoid DC: 9.1 nTPM
memory B-cell: 8.4 nTPM
naive CD4 T-cell: 8.2 nTPM
naive CD8 T-cell: 7.9 nTPM
naive B-cell: 6.8 nTPM

Brain region

hypothalamus: 9.1 nTPM
medulla oblongata: 8.8 nTPM
basal ganglia: 8.7 nTPM
white matter: 8.6 nTPM
cerebral cortex: 8.5 nTPM
pons: 8.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.68
gnomAD pLI: 0
gnomAD missense Z: -0.31
DepMap mean gene effect: 0
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

via spliceosome
mRNA splicing

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of U2AF1L4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads U2AF1L4 as an antibody target. Whether an autoantibody or antibody against U2AF1L4 could matter depends on whether native U2AF1L4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

U2AF1L4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label U2AF1L4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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