Seroatlas · Human Serome Atlas

TSSK4

Testis-specific serine/threonine-protein kinase 4

Also known as: C14orf20, STK22E, TSSK4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6SA08
Gene: TSSK4
Ensembl: ENSG00000139908
Chromosome: 14
Canonical length: 328 aa
Protein class: Enzymes, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Cell Junctions
Quaternary structure: Homodimer

OverviewNCBI Gene

This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Canonical amino-acid sequenceUniProt

328 residues, UniProt reviewed canonical sequence.

>Q6SA08|TSSK4
     1  MGKGDVLEAA PTTTAYHSLM DEYGYEVGKA IGHGSYGSVY EAFYTKQKVM VAVKIISKKK
    61  ASDDYLNKFL PREIQVMKVL RHKYLINFYR AIESTSRVYI ILELAQGGDV LEWIQRYGAC
   121  SEPLAGKWFS QLTLGIAYLH SKSIVHRDLK LENLLLDKWE NVKISDFGFA KMVPSNQPVG
   181  CSPSYRQVNC FSHLSQTYCG SFAYACPEIL RGLPYNPFLS DTWSMGVILY TLVVAHLPFD
   241  DTNLKKLLRE TQKEVTFPAN HTISQECKNL ILQMLRQATK RATILDIIKD SWVLKFQPEQ
   301  PTHEIRLLEA MCQLHNTTKQ HQSLQITT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TSSK4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 36 nTPM

Expression across tissuesHPA

Tissue

testis: 36 nTPM
skin: 3.9 nTPM
bone marrow: 3.2 nTPM
retina: 2.9 nTPM
lymph node: 2.5 nTPM
cerebellum: 2 nTPM

Single-cell type

early spermatids: 179 nCPM
late primary spermatocytes: 122 nCPM
late spermatids: 8.8 nCPM
leydig cells: 0.5 nCPM
mast cells: 0.2 nCPM
peritubular myoid cells: 0.2 nCPM

Immune cell

NK-cell: 0.5 nTPM
eosinophil: 0.4 nTPM
gdT-cell: 0.4 nTPM
memory B-cell: 0.3 nTPM
memory CD4 T-cell: 0.3 nTPM
memory CD8 T-cell: 0.3 nTPM

Brain region

cerebellum: 8.7 nTPM
amygdala: 7.9 nTPM
hippocampal formation: 7.6 nTPM
cerebral cortex: 7.5 nTPM
medulla oblongata: 7.5 nTPM
thalamus: 7.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.52
gnomAD pLI: 0
gnomAD missense Z: 1.07
DepMap mean gene effect: -0.21
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Protein kinase domain
Serine/threonine-protein kinase, active site
Protein kinase-like domain superfamily
Protein kinase, ATP binding site
Protein kinase domain
Testis-specific serine/threonine kinase 4, catalytic domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TSSK4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TSSK4 as an antibody target. Whether an autoantibody or antibody against TSSK4 could matter depends on whether native TSSK4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TSSK4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TSSK4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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