Seroatlas · Human Serome Atlas

TMSB4X

Thymosin beta-4

Also known as: TB4X, TMSB4, TYB4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P62328
Gene
TMSB4X
Ensembl
ENSG00000205542
Chromosome
X
Canonical length
44 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Vesicles,Cytosol

OverviewNCBI Gene

This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

44 residues, UniProt reviewed canonical sequence.

>P62328|TMSB4X
     1  MSDKPDMAEI EKFDKSKLKK TETQEKNPLP SKETIEQEKQ AGES

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMSB4X can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.61
Highest tissue expression
10,459 nTPM

Expression across tissuesHPA

Tissue

  • spinal cord: 10,459 nTPM
  • lymph node: 9,761 nTPM
  • bone marrow: 8,229 nTPM
  • spleen: 7,469 nTPM
  • tonsil: 6,765 nTPM
  • lung: 6,098 nTPM

Single-cell type

  • megakaryocytes: 73,790 nCPM
  • platelets: 38,486 nCPM
  • hofbauer cells: 36,356 nCPM
  • kupffer cells: 30,347 nCPM
  • decidual stromal cells: 27,198 nCPM
  • cdc: 22,882 nCPM

Immune cell

  • total PBMC: 86,915 nTPM
  • eosinophil: 51,158 nTPM
  • basophil: 43,123 nTPM
  • non-classical monocyte: 37,735 nTPM
  • neutrophil: 36,081 nTPM
  • classical monocyte: 34,835 nTPM

Brain region

  • white matter: 3,967 nTPM
  • spinal cord: 3,610 nTPM
  • medulla oblongata: 3,474 nTPM
  • basal ganglia: 2,562 nTPM
  • pons: 2,558 nTPM
  • thalamus: 2,274 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.67
gnomAD pLI
0.41
gnomAD missense Z
0.98
DepMap mean gene effect
-0.22
DepMap dependency class
selective

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TMSB4X in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMSB4X as an antibody target. Whether an autoantibody or antibody against TMSB4X could matter depends on whether native TMSB4X is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMSB4X is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMSB4X as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TMSB4X. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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