TAF4

Protein identityUniProt · HPA

UniProt accession

O00268

Gene

TAF4

Ensembl

ENSG00000130699

Chromosome

20

Canonical length

1085 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid, thyroid hormone and vitamin D3 receptors. In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1085 residues, UniProt reviewed canonical sequence.

>O00268|TAF4
     1  MAAGSDLLDE VFFNSEVDEK VVSDLVGSLE SQLAASAAHH HHLAPRTPEV RAAAAGALGN
    61  HVVSGSPAGA AGAGPAAPAE GAPGAAPEPP PAGRARPGGG GPQRPGPPSP RRPLVPAGPA
   121  PPAAKLRPPP EGSAGSCAPV PAAAAVAAGP EPAPAGPAKP AGPAALAARA GPGPGPGPGP
   181  GPGPGPGKPA GPGAAQTLNG SAALLNSHHA AAPAVSLVNN GPAALLPLPK PAAPGTVIQT
   241  PPFVGAAAPP APAAPSPPAA PAPAAPAAAP PPPPPAPATL ARPPGHPAGP PTAAPAVPPP
   301  AAAQNGGSAG AAPAPAPAAG GPAGVSGQPG PGAAAAAPAP GVKAESPKRV VQAAPPAAQT
   361  LAASGPASTA ASMVIGPTMQ GALPSPAAVP PPAPGTPTGL PKGAAGAVTQ SLSRTPTATT
   421  SGIRATLTPT VLAPRLPQPP QNPTNIQNFQ LPPGMVLVRS ENGQLLMIPQ QALAQMQAQA
   481  HAQPQTTMAP RPATPTSAPP VQISTVQAPG TPIIARQVTP TTIIKQVSQA QTTVQPSATL
   541  QRSPGVQPQL VLGGAAQTAS LGTATAVQTG TPQRTVPGAT TTSSAATETM ENVKKCKNFL
   601  STLIKLASSG KQSTETAANV KELVQNLLDG KIEAEDFTSR LYRELNSSPQ PYLVPFLKRS
   661  LPALRQLTPD SAAFIQQSQQ QPPPPTSQAT TALTAVVLSS SVQRTAGKTA ATVTSALQPP
   721  VLSLTQPTQV GVGKQGQPTP LVIQQPPKPG ALIRPPQVTL TQTPMVALRQ PHNRIMLTTP
   781  QQIQLNPLQP VPVVKPAVLP GTKALSAVSA QAAAAQKNKL KEPGGGSFRD DDDINDVASM
   841  AGVNLSEESA RILATNSELV GTLTRSCKDE TFLLQAPLQR RILEIGKKHG ITELHPDVVS
   901  YVSHATQQRL QNLVEKISET AQQKNFSYKD DDRYEQASDV RAQLKFFEQL DQIEKQRKDE
   961  QEREILMRAA KSRSRQEDPE QLRLKQKAKE MQQQELAQMR QRDANLTALA AIGPRKKRKV
  1021  DCPGPGSGAE GSGPGSVVPG SSGVGTPRQF TRQRITRVNL RDLIFCLENE RETSHSLLLY
  1081  KAFLK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TAF4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.63

Highest tissue expression

10 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TAF4.

Disease | AllUniProt

Conditions TAF4 is implicated in, by any mechanism.

• Intellectual developmental disorder, autosomal dominant 73 (MRD73) MIM:620450

Disease | GeneticClinVar

24 pathogenic / likely-pathogenic of 317 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Intellectual developmental disorder, autosomal dominant 73
• Inborn genetic diseases
• Neurodevelopmental disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.08

gnomAD pLI

1

gnomAD missense Z

2.68

DepMap mean gene effect

-0.3

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 17% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• DNA-templated transcription initiation
• mRNA transcription by RNA polymerase II
• ovarian follicle development
• positive regulation of DNA-templated transcription
• positive regulation of transcription initiation by RNA polymerase II
• regulation of DNA repair
• regulation of transcription by RNA polymerase II
• RNA polymerase II preinitiation complex assembly
• transcription by RNA polymerase II
• transcription initiation at RNA polymerase II promoter

Molecular functions

• aryl hydrocarbon receptor binding
• DNA binding
• protein heterodimerization activity

Cellular components

• chromatin
• cytosol
• male germ cell nucleus
• MLL1 complex
• nucleoplasm
• nucleus
• protein-containing complex
• transcription factor TFIID complex
• transcription factor TFTC complex

Protein domainsUniProt · Pfam · InterPro

• TAFH/NHR1
• Transcription initiation factor TFIID component TAF4, C-terminal
• Histone-fold
• TAFH/NHR1 domain superfamily
• Transcription initiation factor TFIID component TAF4
• Transcription initiation factor TFIID component TAF4 family
• NHR1 homology to TAF

KeywordsUniProt

• Host-virus interaction
• Intellectual disability
• Methylation
• Nucleus
• Phosphoprotein
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of TAF4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ATF7
• HTT
• TAF1
• TAF12
• TBP