SUB1

Protein identityUniProt · HPA

UniProt accession

P53999

Gene

SUB1

Ensembl

ENSG00000113387

Chromosome

5

Canonical length

127 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nucleoli

Quaternary structure

Homodimer

OverviewNCBI Gene

Enables several functions, including DNA binding activity; identical protein binding activity; and transcription coactivator activity. Involved in RNA polymerase II promoter clearance; protein homooligomerization; and regulation of nucleobase-containing compound metabolic process. Located in nucleolus and nucleoplasm. Part of transcription regulator complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

127 residues, UniProt reviewed canonical sequence.

>P53999|SUB1
     1  MPKSKELVSS SSSGSDSDSE VDKKLKRKKQ VAPEKPVKKQ KTGETSRALS SSKQSSSSRD
    61  DNMFQIGKMR YVSVRDFKGK VLIDIREYWM DPEGEMKPGR KGISLNPEQW SQLKEQISDI
   121  DDAVRKL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SUB1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.57

Highest tissue expression

237 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.41

gnomAD pLI

0.89

gnomAD missense Z

1.4

DepMap mean gene effect

-0.02

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• positive regulation of transcription initiation by RNA polymerase II
• regulation of transcription by RNA polymerase II
• RNA polymerase II promoter clearance
• negative regulation of DNA metabolic process

Molecular functions

• double-stranded DNA binding
• identical protein binding
• RNA binding
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• single-stranded DNA binding
• transcription coactivator activity

Cellular components

• extracellular exosome
• nucleolus
• nucleoplasm
• nucleus
• transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• Transcriptional coactivator p15 (PC4), C-terminal
• ssDNA-binding transcriptional regulator
• RNA polymerase II transcriptional coactivator Sub1/Tcp4-like
• Transcriptional Coactivator p15 (PC4)

KeywordsUniProt

• Acetylation
• Activator
• DNA-binding
• Isopeptide bond
• Nucleus
• Phosphoprotein
• Transcription
• Transcription regulation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of SUB1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CSTF2
• RPL11