Seroatlas · Human Serome Atlas

SPRYD4

SPRY domain-containing protein 4

Also known as: DKFZp686N0877, SPRY4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8WW59
Gene
SPRYD4
Ensembl
ENSG00000176422
Chromosome
12
Canonical length
207 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

Located in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

207 residues, UniProt reviewed canonical sequence.

>Q8WW59|SPRYD4
     1  MALLFARSLR LCRWGAKRLG VASTEAQRGV SFKLEEKTAH SSLALFRDDT GVKYGLVGLE
    61  PTKVALNVER FREWAVVLAD TAVTSGRHYW EVTVKRSQQF RIGVADVDMS RDSCIGVDDR
   121  SWVFTYAQRK WYTMLANEKA PVEGIGQPEK VGLLLEYEAQ KLSLVDVSQV SVVHTLQTDF
   181  RGPVVPAFAL WDGELLTHSG LEVPEGL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPRYD4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.32
Highest tissue expression
19 nTPM

Expression across tissuesHPA

Tissue

  • liver: 19 nTPM
  • pancreas: 5.7 nTPM
  • cerebellum: 5 nTPM
  • tongue: 4.7 nTPM
  • skeletal muscle: 3.9 nTPM
  • kidney: 3.8 nTPM

Single-cell type

  • hepatocytes: 150 nCPM
  • esophageal suprabasal cells: 69 nCPM
  • esophageal apical cells: 64 nCPM
  • cytotrophoblasts: 45 nCPM
  • esophageal basal cells: 45 nCPM
  • migrating cytotrophoblasts: 43 nCPM

Immune cell

  • plasmacytoid DC: 1.3 nTPM
  • NK-cell: 1.2 nTPM
  • basophil: 1 nTPM
  • classical monocyte: 1 nTPM
  • memory B-cell: 1 nTPM
  • naive B-cell: 1 nTPM

Brain region

  • cerebellum: 11 nTPM
  • cerebral cortex: 8.2 nTPM
  • hypothalamus: 7.9 nTPM
  • basal ganglia: 7.7 nTPM
  • amygdala: 7.4 nTPM
  • hippocampal formation: 6.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.33
gnomAD pLI
0.01
gnomAD missense Z
0.81
DepMap mean gene effect
-0.48
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPRYD4 as an antibody target. Whether an autoantibody or antibody against SPRYD4 could matter depends on whether native SPRYD4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPRYD4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPRYD4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SPRYD4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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