SPP1

Protein identityUniProt · HPA

UniProt accession

P10451

Gene

SPP1

Ensembl

ENSG00000118785

Chromosome

4

Canonical length

314 aa

Protein class

Cancer-related genes, Plasma proteins, Predicted secreted proteins

Subcellular location

Golgi apparatus

Secretome location

Secreted to blood

OverviewNCBI Gene

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Canonical amino-acid sequenceUniProt

314 residues, UniProt reviewed canonical sequence.

>P10451|SPP1
     1  MRIAVICFCL LGITCAIPVK QADSGSSEEK QLYNKYPDAV ATWLNPDPSQ KQNLLAPQNA
    61  VSSEETNDFK QETLPSKSNE SHDHMDDMDD EDDDDHVDSQ DSIDSNDSDD VDDTDDSHQS
   121  DESHHSDESD ELVTDFPTDL PATEVFTPVV PTVDTYDGRG DSVVYGLRSK SKKFRRPDIQ
   181  YPDATDEDIT SHMESEELNG AYKAIPVAQD LNAPSDWDSR GKDSYETSQL DDQSAETHSH
   241  KQSRLYKRKA NDESNEHSDV IDSQELSKVS REFHSHEFHS HEDMLVVDPK SKEEDKHLKF
   301  RISHELDSAS SEVN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Secreted

Secreted

Yes

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.7

Highest tissue expression

3,271 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SPP1.

Disease | AutoantibodyPubMed

Conditions in which antibodies against SPP1 are reported. Each links to that disease's full target list.

• Arthritis, Rheumatoid 3

ReferencesPubMed · IEDB

Publications for SPP1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.6

gnomAD pLI

0

gnomAD missense Z

0.28

DepMap mean gene effect

0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• androgen catabolic process
• biomineral tissue development
• cell adhesion
• cellular response to testosterone stimulus
• decidualization
• embryo implantation
• osteoblast differentiation
• positive regulation of bone resorption
• positive regulation of DNA-templated transcription
• positive regulation of estradiol secretion
• response to 2,3,7,8-tetrachlorodibenzodioxine
• response to macrophage colony-stimulating factor
• response to steroid hormone
• response to vitamin D
• negative regulation of collateral sprouting of intact axon in response to injury

Molecular functions

• calcium ion binding
• cytokine activity
• extracellular matrix binding
• integrin binding
• small molecule binding

Cellular components

• cell projection
• endoplasmic reticulum lumen
• extracellular exosome
• extracellular region
• extracellular space
• Golgi apparatus
• perinuclear region of cytoplasm

Protein domainsUniProt · Pfam · InterPro

• Osteopontin
• Osteopontin, conserved site
• Osteopontin

KeywordsUniProt

• Biomineralization
• Cell adhesion
• Cytokine
• Glycoprotein
• Phosphoprotein
• Proteoglycan
• Secreted
• Sialic acid
• Signal

InteractionsUniProt · HPA

Protein binding partners of SPP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• IFITM3
• SGTA
• UBQLN4