SPO11

Protein identityUniProt · HPA

UniProt accession

Q9Y5K1

Gene

SPO11

Ensembl

ENSG00000054796

Chromosome

20

Canonical length

396 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

396 residues, UniProt reviewed canonical sequence.

>Q9Y5K1|SPO11
     1  MAFAPMGPEA SFFDVLDRHR ESLLAALRRG GREPPTGGSR LASSSEVLAS IENIIQDIIT
    61  SLARNEAPAF TIDNRSSWEN IKFEDSVGLQ MVSHCTTRKI KSDSPKSAQK FSLILKILSM
   121  IYKLVQSNTY ATKRDIYYTD SQLFGNQTVV DNIINDISCM LKVSRRSLHI LSTSKGLIAG
   181  NLRYIEEDGT KVNCTCGATA VAVPSNIQGI RNLVTDAKFV LIVEKDATFQ RLLDDNFCNK
   241  LSPCIMITGK GVPDLNTRLL VKKLWDTFHV PVFTLVDADP HGIEIMCIYK YGSMSMSFEA
   301  HHLTVPAIRW LGLLPSDLKR LNVPKDSLIP LTKRDQMKLD SILRRPYVTC QPFWRKEMEI
   361  MADSKMKAEI QALTFLSSDY LSRVYLPNKL KFGGWI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPO11 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

7.9 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SPO11.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 43 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Non-obstructive azoospermia

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.11

gnomAD pLI

0

gnomAD missense Z

0.97

DepMap mean gene effect

0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• double-strand break repair involved in meiotic recombination
• female gamete generation
• male meiosis I
• meiotic DNA double-strand break formation
• meiotic telomere clustering
• oogenesis
• ovarian follicle development
• protein localization to chromosome
• reciprocal meiotic recombination
• spermatid development
• spermatogenesis
• synaptonemal complex assembly
• meiotic DNA double-strand break processing

Molecular functions

• ATP binding
• DNA binding
• DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• metal ion binding

Cellular components

• chromosome, telomeric region
• nuclear chromosome

Protein domainsUniProt · Pfam · InterPro

• Winged helix-like DNA-binding domain superfamily
• Spo11/DNA topoisomerase VI subunit A
• Meiosis-specific protein Spo11
• Meiotic recombination, Spo11
• Spo11/DNA topoisomerase VI, subunit A, N-terminal
• Topoisomerase 6 subunit A/Spo11, TOPRIM domain
• Spo11/DNA topoisomerase VI subunit A superfamily
• SPO11 homologue
• Type IIB DNA topoisomerase
• Topoisomerase 6 subunit A/Spo11, Toprim domain

KeywordsUniProt

• DNA-binding
• Isomerase
• Magnesium
• Meiosis
• Metal-binding
• Nucleus
• Topoisomerase

InteractionsUniProt · HPA

Protein binding partners of SPO11 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TOP6BL