Seroatlas · Human Serome Atlas

SPINT4

Kunitz-type protease inhibitor 4

Also known as: C20orf137, dJ601O1.1, SPIT4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6UDR6
Gene
SPINT4
Ensembl
ENSG00000149651
Chromosome
20
Canonical length
99 aa
Protein class
Predicted secreted proteins
Secretome location
Secreted in male reproductive system

OverviewNCBI Gene

Predicted to enable serine-type endopeptidase inhibitor activity. Predicted to be located in extracellular region. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

99 residues, UniProt reviewed canonical sequence.

>Q6UDR6|SPINT4
     1  MKSAKLGFLL RFFIFCSLNT LLLGGVNKIA EKICGDLKDP CKLDMNFGSC YEVHFRYFYN
    61  RTSKRCETFV FSGCNGNLNN FKLKIEREVA CVAKYKPPR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPINT4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.42
Highest tissue expression
263 nTPM

Expression across tissuesHPA

Tissue

  • epididymis: 263 nTPM
  • retina: 1 nTPM
  • seminal vesicle: 0.1 nTPM
  • adipose tissue: 0 nTPM
  • adrenal gland: 0 nTPM
  • amygdala: 0 nTPM

Single-cell type

  • epididymal principal cells: 573 nCPM
  • rod photoreceptor cells: 94 nCPM
  • retinal ganglion cells: 12 nCPM
  • epididymal basal cells: 6.7 nCPM
  • cone photoreceptor cells: 6.2 nCPM
  • retinal horizontal cells: 6 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • midbrain: 0.9 nTPM
  • spinal cord: 0.9 nTPM
  • white matter: 0.6 nTPM
  • medulla oblongata: 0.5 nTPM
  • pons: 0.4 nTPM
  • basal ganglia: 0.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.38
gnomAD pLI
0.08
gnomAD missense Z
0.33
DepMap mean gene effect
0.15
DepMap dependency class
none

OntologyGO

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPINT4 as an antibody target. Whether an autoantibody or antibody against SPINT4 could matter depends on whether native SPINT4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPINT4 is annotated as secreted, so native SPINT4 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label SPINT4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SPINT4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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