Seroatlas · Human Serome Atlas

SPDYE4

Speedy protein E4

Also known as: SPDE4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A6NLX3
Gene: SPDYE4
Ensembl: ENSG00000183318
Chromosome: 17
Canonical length: 237 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable protein kinase binding activity. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

237 residues, UniProt reviewed canonical sequence.

>A6NLX3|SPDYE4
     1  MASGQARPPF EEESPQPSTT VRSPEVVVDD EVPGPSAPWI DPSPQPQSLG LKRKSEWSDE
    61  SEEELEEELE LERAPEPEDT WVVETLCGLK MKLKRKRASS VLPEHHEAFN RLLGDPVVQK
   121  FLAWDKDLRV SDKYLLAMVI AYFSRAGLFS WQYQRIHFFL ALYLASDMEE DNQAPKQDIF
   181  SFLYGKNYSQ RPLFHKLRYQ LLCSMRWRTW VSPEEMEEIQ AYDPEHWVWA RDRTLIS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPDYE4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.43
Highest tissue expression: 5.2 nTPM

Expression across tissuesHPA

Tissue

testis: 5.2 nTPM
bone marrow: 0.7 nTPM
duodenum: 0.3 nTPM
appendix: 0.2 nTPM
cerebral cortex: 0.2 nTPM
esophagus: 0.2 nTPM

Single-cell type

late primary spermatocytes: 61 nCPM
early spermatids: 34 nCPM
late spermatids: 6 nCPM
mast cells: 3.1 nCPM
thymocytes: 1.7 nCPM
granulosa cells: 0.8 nCPM

Immune cell

classical monocyte: 0.1 nTPM
eosinophil: 0.1 nTPM
gdT-cell: 0.1 nTPM
naive B-cell: 0.1 nTPM
naive CD4 T-cell: 0.1 nTPM
naive CD8 T-cell: 0.1 nTPM

Brain region

cerebral cortex: 3.8 nTPM
white matter: 3.6 nTPM
amygdala: 3.4 nTPM
hypothalamus: 3.4 nTPM
cerebellum: 3.1 nTPM
pons: 2.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.86
gnomAD pLI: 0
gnomAD missense Z: 0.34
DepMap mean gene effect: -0.02
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

protein kinase binding

Protein domainsUniProt · Pfam · InterPro

InteractionsUniProt · HPA

Protein binding partners of SPDYE4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPDYE4 as an antibody target. Whether an autoantibody or antibody against SPDYE4 could matter depends on whether native SPDYE4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPDYE4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPDYE4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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