Seroatlas · Human Serome Atlas

SHISA3

Protein shisa-3 homolog

Also known as: hShisa3, SHSA3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A0PJX4
Gene: SHISA3
Ensembl: ENSG00000178343
Chromosome: 4
Canonical length: 238 aa
Protein class: Predicted membrane proteins
Subcellular location: Lipid droplets,Cytosol

OverviewNCBI Gene

This gene encodes a single-transmembrane protein which is one of nine members of a family of transmembrane adaptors that modulate both WNT and FGF signaling by blocking the maturation and transport of their receptors to the cell surface. Members of this family contain an N-terminal cysteine-rich domain with a distinct cysteine pattern, a single transmembrane domain, and a C-terminal proline-rich, low complexity region. The encoded protein acts as a tumor suppressor by accelerating beta-catenin degradation. [provided by RefSeq, Jul 2017]

Canonical amino-acid sequenceUniProt

238 residues, UniProt reviewed canonical sequence.

>A0PJX4|SHISA3
     1  MRALLALCLL LGWLRWGPAG AQQSGEYCHG WVDVQGNYHE GFQCPEDFDT LDATICCGSC
    61  ALRYCCAAAD ARLEQGGCTN DRRELEHPGI TAQPVYVPFL IVGSIFIAFI ILGSVVAIYC
   121  CTCLRPKEPS QQPIRFSLRS YQTETLPMIL TSTSPRAPSR QSSTATSSSS TGGSIRRFSF
   181  ARAEPGCLVP SPPPPYTTSH SIHLAQPSGF LVSPQYFAYP LQQEPPLPGK SCPDFSSS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SHISA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.6
Highest tissue expression: 19 nTPM

Expression across tissuesHPA

Tissue

kidney: 19 nTPM
heart muscle: 14 nTPM
small intestine: 9.2 nTPM
midbrain: 8.6 nTPM
blood vessel: 7.5 nTPM
lymph node: 6.6 nTPM

Single-cell type

epididymal efferent duct absorptive cells: 88 nCPM
decidual stromal cells: 23 nCPM
podocytes: 18 nCPM
fibroblasts: 15 nCPM
distal convoluted tubule cells: 15 nCPM
loop of henle epithelial cells: 13 nCPM

Immune cell

naive B-cell: 0.9 nTPM
memory B-cell: 0.4 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM

Brain region

midbrain: 29 nTPM
thalamus: 20 nTPM
pons: 4.4 nTPM
hypothalamus: 3.3 nTPM
amygdala: 2.5 nTPM
medulla oblongata: 2.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.66
gnomAD pLI: 0
gnomAD missense Z: 0.39
DepMap mean gene effect: 0.08
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

negative regulation of canonical Wnt signaling pathway

Cellular components

endoplasmic reticulum membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SHISA3 as an antibody target. Whether an autoantibody or antibody against SHISA3 could matter depends on whether native SHISA3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SHISA3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SHISA3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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