Seroatlas · Human Serome Atlas

SH3RF3

E3 ubiquitin-protein ligase SH3RF3

Also known as: FLJ00204, POSH2, SH3MD4, SH3R3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8TEJ3
Gene
SH3RF3
Ensembl
ENSG00000172985
Chromosome
2
Canonical length
882 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

Enables ubiquitin protein ligase activity. Involved in positive regulation of JNK cascade and protein autoubiquitination. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

882 residues, UniProt reviewed canonical sequence.

>Q8TEJ3|SH3RF3
     1  MLLGASWLCA SKAAAAAAQS EGDEDRPGER RRRRAAATAA GAGEDMDESS LLDLLECSVC
    61  LERLDTTAKV LPCQHTFCRR CLESIVCSRH ELRCPECRIL VGCGVDELPA NILLVRLLDG
   121  IRQRPRAGTS PGGSPPARPI PGQSAAPTLA GGGGGAAGST PGSPVFLSAA AGSTAGSLRE
   181  LATSRTAPAA KNPCLLPYGK ALYSYEGKEP GDLKFNKGDI IVLRRKVDEQ WYHGELHGTQ
   241  GFLPASYIQC IQPLPHAPPQ GKALYDFEMK DKDQDKDCLT FTKDEILTVL RRVDENWAEG
   301  MLGDKIGIFP LLYVELNDSA KQLIEMDKPC PAAASSCNAS LPSDSGAVAS VAPSPTLSSS
   361  GAVSAFQRRV DGKKNTKKRH SFTALSVTHR SSQAASHRHS MEISAPVLIS SSDPRAAARI
   421  GDLAHLSCAA PTQDVSSSAG STPTAVPRAA SVSGEQGTPP KVQLPLNVYL ALYAYKPQKS
   481  DELELHKGEM YRVLEKCQDG WFKGASLRTG VSGVFPGNYV TPVSRVPAGG AGPPRNNVVG
   541  GSPLAKGITT TMHPGSGSLS SLATATRPAL PITTPQAHAQ HPTASPPTGS CLRHSAQPTA
   601  SQARSTISTA AHSAAQAQDR PTATVSPLRT QNSPSRLPAT SLRPHSVVSP QHSHQPPVQM
   661  CPRPAIPLTS AASAITPPNV SAANLNGEAG GGPIGVLSTS SPTNTGCKLD EKKSEKKEKK
   721  SGLLKLLAGA STKKKSRSPP SVSPTHDPQV AVDALLQGAV GPEVSSLSIH GRAGSCPIES
   781  EMQGAMGMEP LHRKAGSLDL NFTSPSRQAP LSMAAIRPEP KLLPRERYRV VVSYPPQSEA
   841  EIELKEGDIV FVHKKREDGW YKGTLQRNGR TGLFPGSFVE SF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SH3RF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Unknown
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.57
Highest tissue expression
14 nTPM

Expression across tissuesHPA

Tissue

  • blood vessel: 14 nTPM
  • lung: 6.8 nTPM
  • tongue: 6.8 nTPM
  • adrenal gland: 6.3 nTPM
  • adipose tissue: 5.6 nTPM
  • heart muscle: 5.5 nTPM

Single-cell type

  • microglia: 628 nCPM
  • vascular smooth muscle cells: 287 nCPM
  • vascular endothelial cells: 221 nCPM
  • endometrial stromal cells: 200 nCPM
  • fibro-adipogenic progenitors: 199 nCPM
  • retinal horizontal cells: 166 nCPM

Immune cell

  • neutrophil: 0.2 nTPM
  • eosinophil: 0.1 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM

Brain region

  • cerebral cortex: 16 nTPM
  • hippocampal formation: 16 nTPM
  • basal ganglia: 15 nTPM
  • midbrain: 12 nTPM
  • amygdala: 11 nTPM
  • hypothalamus: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.53
gnomAD pLI
0.03
gnomAD missense Z
1.53

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SH3RF3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SH3RF3 as an antibody target. Whether an autoantibody or antibody against SH3RF3 could matter depends on whether native SH3RF3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SH3RF3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SH3RF3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SH3RF3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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