SCAF8

Protein identityUniProt · HPA

UniProt accession

Q9UPN6

Gene

SCAF8

Ensembl

ENSG00000213079

Chromosome

6

Canonical length

1271 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

Enables RNA binding activity and RNA polymerase II C-terminal domain phosphoserine binding activity. Involved in negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled and positive regulation of DNA-templated transcription, elongation. Located in nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1271 residues, UniProt reviewed canonical sequence.

>Q9UPN6|SCAF8
     1  MEAVKTFNSE LYSLNDYKPP ISKAKMTQIT KAAIKAIKFY KHVVQSVEKF IQKCKPEYKV
    61  PGLYVIDSIV RQSRHQFGQE KDVFAPRFSN NIISTFQNLY RCPGDDKSKI VRVLNLWQKN
   121  NVFKSEIIQP LLDMAAGIPP PVVTPVLAST TTAMSNTPGT PVTPVTPANV VQGLPDPWVS
   181  QITNTDTLAA VAQILQSPQG QQLQQLIQTL QIQQQKPQPS ILQALDAGLV VQLQALTAQL
   241  TAAAAAANTL TPLEQGVSFN KKLMDRFDFG EDSEHSEEPK KEIPASQLSH VSESVNNSIF
   301  HQIAEQLQQQ NLEHLRQQLL EQQQPQKATP QDSQEGTFGS EHSASPSQGS SQQHFLEPEV
   361  NLDDSIDIQQ QDMDIDEGQD GVEEEVFEQE AKKVAVRSRS RTHSRSRSRS PRKRRSRSRS
   421  GSRKRKHRKR SRSRSRERKR KSSRSYSSER RAREREKERQ KKGLPPIRSK TLSVCSTTLW
   481  VGQVDKKATQ QDLTNLFEEF GQIESINMIP PRGCAYVCMV HRQDAFRALQ KLSSGSYKIG
   541  SKVIKIAWAL NKGVKTEYKQ FWDVDLGVTY IPWEKVKVDD LEGFAEGGMI DQETVNTEWE
   601  TVKSSEPVKE TVQTTQSPTP VEKETVVTTQ AEVFPPPVAM LQIPVAPAVP TVSLVPPAFP
   661  VSMPVPPPGF SPIPPPPFLR ASFNPSQPPP GFMPPPVPPP VVPPPTIPPV VPTSLVQPSL
   721  SMTPETVKDV GFGSLVIPGG SVASNLATSA LPAGNVFNAP TKQAEPEEKV PHLIDHQISS
   781  GENTRSVIPN DISSNAAILG GQPPNVTSNS GILGVQRPNV SSNSEILGVR PSNVSSSSGI
   841  IAAQPPNILN NSGILGIQPP SVSNSSGLLG VLPPNIPNNS GLVGVQPPNV PNTPGLLGTQ
   901  PPAGPQNLPP LSIPNQRMPT MPMLDIRPGL IPQAPGPRFP LIQPGIPPQR GIPPPSVLDS
   961  ALHPPPRGPF PPGDIFSQPE RPFLAPGRQS VDNVTNPEKR IPLGNDNIQQ EGDRDYRFPP
  1021  IETRESISRP PPVDVRDVVG RPIDPREGPG RPPLDGRDHF GRPPVDIREN LVRPGIDHLG
  1081  RRDHFGFNPE KPWGHRGDFD EREHRVLPVY GGPKGLHEER GRFRSGNYRF DPRSGPWNRG
  1141  FGQEVHRDFD DRRRPWERQR DRDDRDFDFC REMNGNRLGR DRIQNTWVPP PHARVFDYFE
  1201  GATSQRKGDN VPQVNGENTE RHAQPPPIPV QNDPELYEKL TSSNEINKEK SDTVADIESE
  1261  PVVESTETEG T

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SCAF8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.58

Highest tissue expression

35 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.1

gnomAD pLI

1

gnomAD missense Z

1.9

DepMap mean gene effect

-0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled
• positive regulation of DNA-templated transcription, elongation
• termination of RNA polymerase II transcription

Molecular functions

• mRNA binding
• RNA binding
• RNA polymerase core enzyme binding
• RNA polymerase II C-terminal domain phosphoserine binding
• RNA polymerase II complex binding

Cellular components

• cytoplasm
• mRNA cleavage factor complex
• nuclear matrix
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• RNA recognition motif domain
• CID domain
• ENTH/VHS
• Nucleotide-binding alpha-beta plait domain superfamily
• RNA-binding domain superfamily
• SR-related and CTD-associated factor
• RNA recognition motif
• CID domain
• SCAF8, RNA recognition motif

KeywordsUniProt

• Isopeptide bond
• Methylation
• Nucleus
• Phosphoprotein
• RNA-binding
• Transcription
• Transcription regulation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of SCAF8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• POLR2A