RUBCNL

Protein identityUniProt · HPA

UniProt accession

Q9H714

Gene

RUBCNL

Ensembl

ENSG00000102445

Chromosome

13

Canonical length

662 aa

Protein class

Predicted intracellular proteins

Subcellular location

Cytosol

OverviewNCBI Gene

This gene encodes a cysteine-rich protein that contains a putative zinc-RING and/or ribbon domain. The encoded protein is related to Run domain Beclin-1-interacting and cysteine-rich domain-containing protein, which plays a role in endocytic trafficking and autophagy. In cervical cancer cell lines, this gene is expressed at low levels and may function as a tumor suppressor. Promoter hypermethylation of this gene is observed in cervical cancer cell lines and tissue derived from human patients. [provided by RefSeq, Mar 2017]

Canonical amino-acid sequenceUniProt

662 residues, UniProt reviewed canonical sequence.

>Q9H714|RUBCNL
     1  MVSQSTVRQD SPVEPWEGIS DHSGIIDGSP RLLNTDHPPC QLDIRLMRHK AVWINPQDVQ
    61  QQPQDLQSQV PAAGNSGTHF VTDAASPSGP SPSCLGDSLA ETTLSEDTTD SVGSASPHGS
   121  SEKSSSFSLS STEVHMVRPG YSHRVSLPTS PGILATSPYP ETDSAFFEPS HLTSAADEGA
   181  VQVSRRTISS NSFSPEVFVL PVDVEKENAH FYVADMIISA MEKMKCNILS QQQTESWSKE
   241  VSGLLGSDQP DSEMTFDTNI KQESGSSTSS YSGYEGCAVL QVSPVTETRT YHDVKEICKC
   301  DVDEFVILEL GDFNDITETC SCSCSSSKSV TYEPDFNSAE LLAKELYRVF QKCWILSVVN
   361  SQLAGSLSAA GSIVVNEECV RKDFESSMNV VQEIKFKSRI RGTEDWAPPR FQIIFNIHPP
   421  LKRDLVVAAQ NFFCAGCGTP VEPKFVKRLR YCEYLGKYFC DCCHSYAESC IPARILMMWD
   481  FKKYYVSNFS KQLLDSIWHQ PIFNLLSIGQ SLYAKAKELD RVKEIQEQLF HIKKLLKTCR
   541  FANSALKEFE QVPGHLTDEL HLFSLEDLVR IKKGLLAPLL KDILKASLAH VAGCELCQGK
   601  GFICEFCQNT TVIFPFQTAT CRRCSACRAC FHKQCFQSSE CPRCARITAR RKLLESVASA
   661  AT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RUBCNL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.52

Highest tissue expression

39 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.27

gnomAD pLI

0

DepMap mean gene effect

0.09

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• autophagosome maturation
• autophagosome-lysosome fusion
• lipid metabolic process
• regulation of glycogen metabolic process
• regulation of lipid metabolic process
• autophagosome-endosome fusion

Molecular functions

• phosphatidylinositol phosphate binding
• phosphatidylinositol-3-phosphate binding
• phosphatidylinositol-4-phosphate binding
• phosphatidylinositol-5-phosphate binding

Cellular components

• autophagosome membrane
• cytoplasmic vesicle

Protein domainsUniProt · Pfam · InterPro

• Rubicon Homology Domain
• Rubicon, PI3K-binding domain
• Autophagy and Host Defense Regulator
• Rubicon Homology Domain
• Rubicon, PI3K-binding domain

KeywordsUniProt

• Acetylation
• Autophagy
• Cytoplasmic vesicle
• Lipid metabolism
• Lipid-binding
• Membrane
• Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of RUBCNL in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• STX17
• UVRAG