Seroatlas · Human Serome Atlas

RTRAF

RNA transcription, translation and transport factor protein

Also known as: C14orf166, CGI-99, CLE, CLE7, hCLE1, LCRP369, RLLM1, RTRAF_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y224
Gene: RTRAF
Ensembl: ENSG00000087302
Chromosome: 14
Canonical length: 244 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm
Quaternary structure: Homodimer

OverviewNCBI Gene

Enables RNA binding activity; RNA polymerase II complex binding activity; and identical protein binding activity. Involved in negative regulation of protein kinase activity; positive regulation of transcription by RNA polymerase II; and tRNA splicing, via endonucleolytic cleavage and ligation. Located in microtubule cytoskeleton; nucleoplasm; and perinuclear region of cytoplasm. Part of tRNA-splicing ligase complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

244 residues, UniProt reviewed canonical sequence.

>Q9Y224|RTRAF
     1  MFRRKLTALD YHNPAGFNCK DETEFRNFIV WLEDQKIRHY KIEDRGNLRN IHSSDWPKFF
    61  EKYLRDVNCP FKIQDRQEAI DWLLGLAVRL EYGDNAEKYK DLVPDNSKTA DNATKNAEPL
   121  INLDVNNPDF KAGVMALANL LQIQRHDDYL VMLKAIRILV QERLTQDAVA KANQTKEGLP
   181  VALDKHILGF DTGDAVLNEA AQILRLLHIE ELRELQTKIN EAIVAVQAII ADPKTDHRLG
   241  KVGR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RTRAF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.43
Highest tissue expression: 150 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 150 nTPM
heart muscle: 143 nTPM
testis: 133 nTPM
bone marrow: 131 nTPM
pancreas: 108 nTPM
thymus: 100 nTPM

Single-cell type

late primary spermatocytes: 1,027 nCPM
oocytes: 843 nCPM
late spermatids: 825 nCPM
early spermatids: 598 nCPM
esophageal suprabasal cells: 430 nCPM
extravillous trophoblasts: 426 nCPM

Immune cell

T-reg: 144 nTPM
total PBMC: 127 nTPM
myeloid DC: 118 nTPM
non-classical monocyte: 113 nTPM
memory B-cell: 109 nTPM
plasmacytoid DC: 108 nTPM

Brain region

white matter: 40 nTPM
choroid plexus: 37 nTPM
spinal cord: 35 nTPM
hypothalamus: 34 nTPM
cerebellum: 33 nTPM
medulla oblongata: 33 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.59
gnomAD pLI: 0.2
DepMap mean gene effect: -0.38
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

RNA transcription, translation and transport factor protein
RNA transcription, translation and transport factor protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RTRAF in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RTRAF as an antibody target. Whether an autoantibody or antibody against RTRAF could matter depends on whether native RTRAF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RTRAF is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RTRAF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...