RO60

Protein identityUniProt · HPA

UniProt accession

P10155

Gene

RO60

Ensembl

ENSG00000116747

Chromosome

1

Canonical length

538 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear bodies,Primary cilium,Cytosol

OverviewNCBI Gene

Enables RNA binding activity. Involved in cellular response to interferon-alpha and regulation of gene expression. Located in cilium; cytosol; and nuclear body. Part of ribonucleoprotein complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

538 residues, UniProt reviewed canonical sequence.

>P10155|RO60
     1  MEESVNQMQP LNEKQIANSQ DGYVWQVTDM NRLHRFLCFG SEGGTYYIKE QKLGLENAEA
    61  LIRLIEDGRG CEVIQEIKSF SQEGRTTKQE PMLFALAICS QCSDISTKQA AFKAVSEVCR
   121  IPTHLFTFIQ FKKDLKESMK CGMWGRALRK AIADWYNEKG GMALALAVTK YKQRNGWSHK
   181  DLLRLSHLKP SSEGLAIVTK YITKGWKEVH ELYKEKALSV ETEKLLKYLE AVEKVKRTRD
   241  ELEVIHLIEE HRLVREHLLT NHLKSKEVWK ALLQEMPLTA LLRNLGKMTA NSVLEPGNSE
   301  VSLVCEKLCN EKLLKKARIH PFHILIALET YKTGHGLRGK LKWRPDEEIL KALDAAFYKT
   361  FKTVEPTGKR FLLAVDVSAS MNQRVLGSIL NASTVAAAMC MVVTRTEKDS YVVAFSDEMV
   421  PCPVTTDMTL QQVLMAMSQI PAGGTDCSLP MIWAQKTNTP ADVFIVFTDN ETFAGGVHPA
   481  IALREYRKKM DIPAKLIVCG MTSNGFTIAD PDDRGMLDMC GFDTGALDVI RNFTLDMI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RO60 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

28 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RO60.

Disease | ImmuneIEDB

Conditions an epitope on RO60 was assayed in.

• systemic scleroderma B cell
• systemic lupus erythematosus B cell

Disease | AutoantibodyPubMed

Conditions in which antibodies against RO60 are reported. Each links to that disease's full target list.

• Sjogren's Syndrome 236
• Lupus Erythematosus, Systemic 158
• Heart Block 47
• Scleroderma, Systemic 25
• Arthritis, Rheumatoid 20
• Myositis 19
• Lung Diseases, Interstitial 17
• Lupus Erythematosus, Cutaneous 17
• Lupus Nephritis 12
• Neuromyelitis Optica 9
• Liver Cirrhosis, Biliary 7
• Lupus Erythematosus, Discoid 7
• Hepatitis, Autoimmune 6
• Mixed Connective Tissue Disease 6
• Dermatomyositis 5
• Thrombocytopenia 5
• Abortion, Spontaneous 4
• Antiphospholipid Syndrome 4
• Hypergammaglobulinemia 4
• Sarcoidosis 4

Showing 20 of 42 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for RO60 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.43

gnomAD pLI

0.58

DepMap mean gene effect

-0.05

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to interferon-alpha
• cilium assembly
• immune system development
• regulation of gene expression
• response to UV
• smoothened signaling pathway
• transcription by RNA polymerase III

Molecular functions

• metal ion binding
• RNA binding
• U2 snRNA binding

Cellular components

• cilium
• cytosol
• nuclear body
• nucleoplasm
• ribonucleoprotein complex

Protein domainsUniProt · Pfam · InterPro

• TROVE domain
• von Willebrand factor A-like domain superfamily
• TROVE domain superfamily
• TROVE domain
• RNA-binding protein RO60
• RNA-binding protein RO60, vWA domain
• Ro60 vWA domain

KeywordsUniProt

• Acetylation
• Cilium biogenesis/degradation
• Cytoplasm
• Metal-binding
• Phosphoprotein
• Repeat
• Ribonucleoprotein
• RNA-binding

InteractionsUniProt · HPA

Protein binding partners of RO60 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RAB11FIP5
• PUF60
• SSB
• U2AF2