Seroatlas · Human Serome Atlas

RNF169

E3 ubiquitin-protein ligase RNF169

Also known as: KIAA1991, RN169_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8NCN4
Gene
RNF169
Ensembl
ENSG00000166439
Chromosome
11
Canonical length
708 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli,Nucleoli rim,Nuclear bodies,Cytosol

OverviewNCBI Gene

Enables K63-linked polyubiquitin modification-dependent protein binding activity; nucleosome binding activity; and ubiquitin-modified histone reader activity. Involved in double-strand break repair via homologous recombination and negative regulation of double-strand break repair. Located in cytosol; nuclear lumen; and site of double-strand break. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

708 residues, UniProt reviewed canonical sequence.

>Q8NCN4|RNF169
     1  MAAAGPSTRA SSAAAAAALS RRGRRGRCDE TAAAKTGAPG PASGPSLLVL SPPLLQPPLP
    61  PRPEESGCAG CLEPPGEAAA LPCGHSLCRG CAQRAADAAG PGCPRCRARG PGWARRRARD
   121  DGQADSEVLG ECARRSQPER CRPRRDGGAA AAGPRPEQEP RAAPAEPDFI FRAPIKLSKP
   181  GELREEYESL RKLREEKLQE EKPSEDQIHK LLPEDTETGK RKMDEQKKRD EPLVLKTNLE
   241  RCPARLSDSE NEEPSRGQMT QTHRSAFVSK NNSYSLAFLA GKLNSKVERS QSCSDTAQER
   301  AKSRVRAVPG NKAKVTTMTP ASNPIIGVLL STQNNRCVSA PDLTIEKRLP FSSLSSLASL
   361  HKPERSVSPE SNDSISEELN HFKPIVCSPC TPPKRLPDGR VLSPLIIKST PRNLNRSLQK
   421  QTSYEASPRI LKKWEQIFQE RQIKKTLSKA TLTSLAPEMG EELLGSEGIH SSKEKPLVAV
   481  NTRLSGGQVL SEYTGPTSAD LDHFPSVSQT KAEQDSDNKS STEIPLETCC SSELKGGGSG
   541  TSLEREQFEG LGSTPDAKLD KTCISRAMKI TTVNSVLPQN SVLGGVLKTK QQLKTLNHFD
   601  LTNGVLVESL SEEPLPSLRR GRKRHCKTKH LEQNGSLKKL RQTSGEVGLA PTDPVLREME
   661  QKLQQEEEDR QLALQLQRMF DNERRTVSRR KGSVDQYLLR SSNMAGAK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF169 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.66
Highest tissue expression
14 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 14 nTPM
  • esophagus: 9.5 nTPM
  • endometrium: 7.2 nTPM
  • thymus: 7.2 nTPM
  • smooth muscle: 7.1 nTPM
  • spleen: 6.9 nTPM

Single-cell type

  • esophageal apical cells: 381 nCPM
  • neutrophils: 196 nCPM
  • myonuclei: 196 nCPM
  • cardiomyocytes: 156 nCPM
  • megakaryocyte-erythroid progenitors: 154 nCPM
  • epicardial cells: 143 nCPM

Immune cell

  • neutrophil: 1.4 nTPM
  • basophil: 1.2 nTPM
  • classical monocyte: 0.5 nTPM
  • eosinophil: 0.4 nTPM
  • gdT-cell: 0.4 nTPM
  • memory CD4 T-cell: 0.4 nTPM

Brain region

  • white matter: 27 nTPM
  • cerebellum: 22 nTPM
  • basal ganglia: 21 nTPM
  • medulla oblongata: 20 nTPM
  • choroid plexus: 20 nTPM
  • midbrain: 19 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.55
gnomAD pLI
0.04
gnomAD missense Z
0.36
DepMap mean gene effect
-0.12
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF169 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF169 as an antibody target. Whether an autoantibody or antibody against RNF169 could matter depends on whether native RNF169 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF169 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF169 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RNF169. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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