Seroatlas · Human Serome Atlas

RNF149

E3 ubiquitin-protein ligase RNF149

Also known as: FLJ90504, RN149_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NC42
Gene: RNF149
Ensembl: ENSG00000163162
Chromosome: 2
Canonical length: 400 aa
Protein class: Enzymes, Metabolic proteins, Predicted membrane proteins
Subcellular location: Vesicles,Plasma membrane

OverviewNCBI Gene

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in ubiquitin-dependent protein catabolic process. Predicted to act upstream of or within cellular response to xenobiotic stimulus; negative regulation of MAPK cascade; and regulation of protein stability. Located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

400 residues, UniProt reviewed canonical sequence.

>Q8NC42|RNF149
     1  MAWRRREASV GARGVLALAL LALALCVPGA RGRALEWFSA VVNIEYVDPQ TNLTVWSVSE
    61  SGRFGDSSPK EGAHGLVGVP WAPGGDLEGC APDTRFFVPE PGGRGAAPWV ALVARGGCTF
   121  KDKVLVAARR NASAVVLYNE ERYGNITLPM SHAGTGNIVV IMISYPKGRE ILELVQKGIP
   181  VTMTIGVGTR HVQEFISGQS VVFVAIAFIT MMIISLAWLI FYYIQRFLYT GSQIGSQSHR
   241  KETKKVIGQL LLHTVKHGEK GIDVDAENCA VCIENFKVKD IIRILPCKHI FHRICIDPWL
   301  LDHRTCPMCK LDVIKALGYW GEPGDVQEMP APESPPGRDP AANLSLALPD DDGSDDSSPP
   361  SASPAESEPQ CDPSFKGDAG ENTALLEAGR SDSRHGGPIS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF149 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 49 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 49 nTPM
spleen: 44 nTPM
appendix: 38 nTPM
esophagus: 28 nTPM
urinary bladder: 27 nTPM
lung: 26 nTPM

Single-cell type

neutrophils: 6,808 nCPM
neutrophil progenitors: 1,143 nCPM
monocytes: 990 nCPM
kupffer cells: 973 nCPM
macrophages: 600 nCPM
pdcs: 591 nCPM

Immune cell

neutrophil: 621 nTPM
non-classical monocyte: 135 nTPM
intermediate monocyte: 118 nTPM
eosinophil: 91 nTPM
classical monocyte: 74 nTPM
total PBMC: 70 nTPM

Brain region

choroid plexus: 20 nTPM
white matter: 18 nTPM
medulla oblongata: 16 nTPM
pons: 15 nTPM
thalamus: 14 nTPM
hypothalamus: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.85
gnomAD pLI: 0
gnomAD missense Z: 1.14
DepMap mean gene effect: -0.06
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Zinc finger, RING-type
PA domain
Zinc finger, RING/FYVE/PHD-type
PA domain superfamily
PA domain
Ring finger domain
E3 ubiquitin-protein ligase RNF149, RING finger, H2 subclass

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF149 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF149 as an antibody target. Whether an autoantibody or antibody against RNF149 could matter depends on whether native RNF149 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF149 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF149 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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