Seroatlas · Human Serome Atlas

RHOF

Rho-related GTP-binding protein RhoF

Also known as: ARHF, FLJ20247, RHOF_HUMAN, RIF

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9HBH0
Gene: RHOF
Ensembl: ENSG00000139725
Chromosome: 12
Canonical length: 211 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Golgi apparatus

OverviewNCBI Gene

Predicted to enable GTP binding activity; GTPase activity; and protein kinase binding activity. Involved in actin filament organization. Located in extracellular exosome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

211 residues, UniProt reviewed canonical sequence.

>Q9HBH0|RHOF
     1  MDAPGALAQT AAPGPGRKEL KIVIVGDGGC GKTSLLMVYS QGSFPEHYAP SVFEKYTASV
    61  TVGSKEVTLN LYDTAGQEDY DRLRPLSYQN THLVLICYDV MNPTSYDNVL IKWFPEVTHF
   121  CRGIPMVLIG CKTDLRKDKE QLRKLRAAQL EPITYMQGLS ACEQIRAALY LECSAKFREN
   181  VEDVFREAAK VALSALKKAQ RQKKRRLCLL L

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RHOF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 45 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 45 nTPM
lymph node: 38 nTPM
spleen: 31 nTPM
small intestine: 31 nTPM
duodenum: 30 nTPM
tonsil: 29 nTPM

Single-cell type

megakaryocytes: 359 nCPM
platelets: 341 nCPM
enterocytes: 190 nCPM
colonocytes: 165 nCPM
esophageal apical cells: 153 nCPM
alveolar cells type 1: 125 nCPM

Immune cell

gdT-cell: 97 nTPM
MAIT T-cell: 93 nTPM
T-reg: 90 nTPM
memory CD8 T-cell: 82 nTPM
memory CD4 T-cell: 77 nTPM
plasmacytoid DC: 74 nTPM

Brain region

cerebellum: 53 nTPM
pons: 36 nTPM
midbrain: 35 nTPM
cerebral cortex: 33 nTPM
medulla oblongata: 32 nTPM
white matter: 32 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.29
gnomAD pLI: 0
gnomAD missense Z: 0.87
DepMap mean gene effect: -0.28
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RHOF as an antibody target. Whether an autoantibody or antibody against RHOF could matter depends on whether native RHOF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RHOF is annotated at the cell surface, where native RHOF is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label RHOF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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