RGMA

Protein identityUniProt · HPA

UniProt accession

Q96B86

Gene

RGMA

Ensembl

ENSG00000182175

Chromosome

15

Canonical length

450 aa

Protein class

Predicted intracellular proteins, Predicted secreted proteins

Subcellular location

Nucleoplasm,Cytosol

Secretome location

Secreted - unknown location

OverviewNCBI Gene

This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Canonical amino-acid sequenceUniProt

450 residues, UniProt reviewed canonical sequence.

>Q96B86|RGMA
     1  MQPPRERLVV TGRAGWMGMG RGAGRSALGF WPTLAFLLCS FPAATSPCKI LKCNSEFWSA
    61  TSGSHAPASD DTPEFCAALR SYALCTRRTA RTCRGDLAYH SAVHGIEDLM SQHNCSKDGP
   121  TSQPRLRTLP PAGDSQERSD SPEICHYEKS FHKHSATPNY THCGLFGDPH LRTFTDRFQT
   181  CKVQGAWPLI DNNYLNVQVT NTPVLPGSAA TATSKLTIIF KNFQECVDQK VYQAEMDELP
   241  AAFVDGSKNG GDKHGANSLK ITEKVSGQHV EIQAKYIGTT IVVRQVGRYL TFAVRMPEEV
   301  VNAVEDWDSQ GLYLCLRGCP LNQQIDFQAF HTNAEGTGAR RLAAASPAPT APETFPYETA
   361  VAKCKEKLPV EDLYYQACVF DLLTTGDVNF TLAAYYALED VKMLHSNKDK LHLYERTRDL
   421  PGRAAAGLPL APRPLLGALV PLLALLPVFC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RGMA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.37

Highest tissue expression

189 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for RGMA from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.62

gnomAD pLI

0.16

gnomAD missense Z

0.99

DepMap mean gene effect

0

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• BMP signaling pathway
• membrane protein ectodomain proteolysis
• negative regulation of axon regeneration
• negative regulation of collateral sprouting
• neural tube closure
• neuron projection development
• positive regulation of membrane protein ectodomain proteolysis
• positive regulation of neuron projection development
• positive regulation of transcription by RNA polymerase II
• regulation of BMP signaling pathway

Molecular functions

• coreceptor activity
• transferrin receptor binding

Cellular components

• cell surface
• endoplasmic reticulum
• plasma membrane
• side of membrane

Protein domainsUniProt · Pfam · InterPro

• Repulsive guidance molecule, C-terminal
• Repulsive guidance molecule, N-terminal
• Repulsive guidance molecule
• Repulsive guidance molecule (RGM) C-terminal domain
• Repulsive guidance molecule (RGM) N-terminal domain

KeywordsUniProt

• Autocatalytic cleavage
• Cell membrane
• Disulfide bond
• Glycoprotein
• GPI-anchor
• Lipoprotein
• Membrane
• Signal

InteractionsUniProt · HPA

Protein binding partners of RGMA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• BMP2
• BMP4
• NEO1