Seroatlas · Human Serome Atlas

RASGRF2

Ras-specific guanine nucleotide-releasing factor 2

Also known as: GRF2, Ras-GRF2, RGRF2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O14827
Gene: RASGRF2
Ensembl: ENSG00000113319
Chromosome: 5
Canonical length: 1237 aa
Protein class: Predicted intracellular proteins, RAS pathway related proteins
Subcellular location: Endoplasmic reticulum
Quaternary structure: Homooligomer

OverviewNCBI Gene

RAS GTPases cycle between an inactive GDP-bound state and an active GTP-bound state. This gene encodes a calcium-regulated nucleotide exchange factor activating both RAS and RAS-related protein, RAC1, through the exchange of bound GDP for GTP, thereby, coordinating the signaling of distinct mitogen-activated protein kinase pathways. [provided by RefSeq, Oct 2011]

Canonical amino-acid sequenceUniProt

1237 residues, UniProt reviewed canonical sequence.

>O14827|RASGRF2
     1  MQKSVRYNEG HALYLAFLAR KEGTKRGFLS KKTAEASRWH EKWFALYQNV LFYFEGEQSC
    61  RPAGMYLLEG CSCERTPAPP RAGAGQGGVR DALDKQYYFT VLFGHEGQKP LELRCEEEQD
   121  GKEWMEAIHQ ASYADILIER EVLMQKYIHL VQIVETEKIA ANQLRHQLED QDTEIERLKS
   181  EIIALNKTKE RMRPYQSNQE DEDPDIKKIK KVQSFMRGWL CRRKWKTIVQ DYICSPHAES
   241  MRKRNQIVFT MVEAESEYVH QLYILVNGFL RPLRMAASSK KPPISHDDVS SIFLNSETIM
   301  FLHEIFHQGL KARIANWPTL ILADLFDILL PMLNIYQEFV RNHQYSLQVL ANCKQNRDFD
   361  KLLKQYEANP ACEGRMLETF LTYPMFQIPR YIITLHELLA HTPHEHVERK SLEFAKSKLE
   421  ELSRVMHDEV SDTENIRKNL AIERMIVEGC DILLDTSQTF IRQGSLIQVP SVERGKLSKV
   481  RLGSLSLKKE GERQCFLFTK HFLICTRSSG GKLHLLKTGG VLSLIDCTLI EEPDASDDDS
   541  KGSGQVFGHL DFKIVVEPPD AAAFTVVLLA PSRQEKAAWM SDISQCVDNI RCNGLMTIVF
   601  EENSKVTVPH MIKSDARLHK DDTDICFSKT LNSCKVPQIR YASVERLLER LTDLRFLSID
   661  FLNTFLHTYR IFTTAAVVLG KLSDIYKRPF TSIPVRSLEL FFATSQNNRG EHLVDGKSPR
   721  LCRKFSSPPP LAVSRTSSPV RARKLSLTSP LNSKIGALDL TTSSSPTTTT QSPAASPPPH
   781  TGQIPLDLSR GLSSPEQSPG TVEENVDNPR VDLCNKLKRS IQKAVLESAP ADRAGVESSP
   841  AADTTELSPC RSPSTPRHLR YRQPGGQTAD NAHCSVSPAS AFAIATAAAG HGSPPGFNNT
   901  ERTCDKEFII RRTATNRVLN VLRHWVSKHA QDFELNNELK MNVLNLLEEV LRDPDLLPQE
   961  RKAAANILRA LSQDDQDDIH LKLEDIIQMT DCMKAECFES LSAMELAEQI TLLDHVIFRS
  1021  IPYEEFLGQG WMKLDKNERT PYIMKTSQHF NDMSNLVASQ IMNYADVSSR ANAIEKWVAV
  1081  ADICRCLHNY NGVLEITSAL NRSAIYRLKK TWAKVSKQTK ALMDKLQKTV SSEGRFKNLR
  1141  ETLKNCNPPA VPYLGMYLTD LAFIEEGTPN FTEEGLVNFS KMRMISHIIR EIRQFQQTSY
  1201  RIDHQPKVAQ YLLDKDLIID EDTLYELSLK IEPRLPA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RASGRF2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.36
Highest tissue expression: 21 nTPM

Expression across tissuesHPA

Tissue

retina: 21 nTPM
cerebral cortex: 18 nTPM
spinal cord: 15 nTPM
ovary: 15 nTPM
choroid plexus: 13 nTPM
adipose tissue: 11 nTPM

Single-cell type

choroid plexus epithelial cells: 845 nCPM
cone photoreceptor cells: 634 nCPM
rod photoreceptor cells: 578 nCPM
oligodendrocytes: 424 nCPM
extravillous trophoblasts: 372 nCPM
vascular endothelial cells: 302 nCPM

Immune cell

MAIT T-cell: 5.2 nTPM
naive CD4 T-cell: 4.2 nTPM
naive CD8 T-cell: 3 nTPM
memory CD4 T-cell: 2.6 nTPM
gdT-cell: 1.9 nTPM
memory CD8 T-cell: 1.5 nTPM

Brain region

choroid plexus: 44 nTPM
cerebral cortex: 40 nTPM
hypothalamus: 38 nTPM
amygdala: 32 nTPM
hippocampal formation: 30 nTPM
white matter: 29 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.36
gnomAD pLI: 0.15
gnomAD missense Z: 3.03
DepMap mean gene effect: -0.05
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RASGRF2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RASGRF2 as an antibody target. Whether an autoantibody or antibody against RASGRF2 could matter depends on whether native RASGRF2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RASGRF2 is annotated at the cell surface, where native RASGRF2 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label RASGRF2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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