Seroatlas · Human Serome Atlas

PUS10

tRNA pseudouridine synthase Pus10

Also known as: CCDC139, FLJ32312, PUS10_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q3MIT2
Gene: PUS10
Ensembl: ENSG00000162927
Chromosome: 2
Canonical length: 529 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies,Mitochondria

OverviewNCBI Gene

Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009]

Canonical amino-acid sequenceUniProt

529 residues, UniProt reviewed canonical sequence.

>Q3MIT2|PUS10
     1  MFPLTEENKH VAQLLLNTGT CPRCIFRFCG VDFHAPYKLP YKELLNELQK FLETEKDELI
    61  LEVMNPPPKK IRLQELEDSI DNLSQNGEGR ISVSHVGSTA SKNSNLNVCN VCLGILQEFC
   121  EKDFIKKVCQ KVEASGFEFT SLVFSVSFPP QLSVREHAAW LLVKQEMGKQ SLSLGRDDIV
   181  QLKEAYKWIT HPLFSEELGV PIDGKSLFEV SVVFAHPETV EDCHFLAAIC PDCFKPAKNK
   241  QSVFTRMAVM KALNKIKEED FLKQFPCPPN SPKAVCAVLE IECAHGAVFV AGRYNKYSRN
   301  LPQTPWIIDG ERKLESSVEE LISDHLLAVF KAESFNFSSS GREDVDVRTL GNGRPFAIEL
   361  VNPHRVHFTS QEIKELQQKI NNSSNKIQVR DLQLVTREAI GHMKEGEEEK TKTYSALIWT
   421  NKAIQKKDIE FLNDIKDLKI DQKTPLRVLH RRPLAVRARV IHFMETQYVD EHHFRLHLKT
   481  QAGTYIKEFV HGDFGRTKPN IGSLMNVTAD ILELDVESVD VDWPPALDD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PUS10 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.3
Highest tissue expression: 14 nTPM

Expression across tissuesHPA

Tissue

liver: 14 nTPM
small intestine: 8.7 nTPM
duodenum: 7.1 nTPM
thyroid gland: 6.4 nTPM
tonsil: 6.4 nTPM
lymph node: 6.3 nTPM

Single-cell type

epicardial cells: 296 nCPM
cardiomyocytes: 96 nCPM
myonuclei: 93 nCPM
enterocytes: 70 nCPM
adipocytes: 56 nCPM
alveolar cells type 2: 53 nCPM

Immune cell

basophil: 9.1 nTPM
non-classical monocyte: 4.8 nTPM
NK-cell: 3.1 nTPM
memory B-cell: 3 nTPM
plasmacytoid DC: 2.9 nTPM
MAIT T-cell: 2.5 nTPM

Brain region

white matter: 7.3 nTPM
choroid plexus: 6.8 nTPM
spinal cord: 6.4 nTPM
thalamus: 6.4 nTPM
medulla oblongata: 6.3 nTPM
cerebral cortex: 6.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.04
gnomAD pLI: 0
gnomAD missense Z: 0.04
DepMap mean gene effect: -0.07
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Pseudouridine synthase, catalytic domain superfamily
tRNA pseudouridine synthase Pus10-like
Pus10-like, C-terminal
Pus10, N-terminal, eukaryotes
Eukaryotic Pus10, N-terminal
Pus10, C-terminal

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PUS10 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PUS10 as an antibody target. Whether an autoantibody or antibody against PUS10 could matter depends on whether native PUS10 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PUS10 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PUS10 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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