PTAFR

Protein identityUniProt · HPA

UniProt accession

P25105

Gene

PTAFR

Ensembl

ENSG00000169403

Chromosome

1

Canonical length

342 aa

Protein class

FDA approved drug targets, G-protein coupled receptors, Predicted membrane proteins, Transporters

Subcellular location

Nucleoplasm,Vesicles,Plasma membrane,Primary cilium

OverviewNCBI Gene

This gene encodes a seven-transmembrane G-protein-coupled receptor for platelet-activating factor (PAF) that localizes to lipid rafts and/or caveolae in the cell membrane. PAF (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a phospholipid that plays a significant role in oncogenic transformation, tumor growth, angiogenesis, metastasis, and pro-inflammatory processes. Binding of PAF to the PAF-receptor (PAFR) stimulates numerous signal transduction pathways including phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs), and the phosphatidylinositol-calcium second messenger system. Following PAFR activation, cells become rapidly desensitized and this refractory state is dependent on PAFR phosphorylation, internalization, and down-regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

342 residues, UniProt reviewed canonical sequence.

>P25105|PTAFR
     1  MEPHDSSHMD SEFRYTLFPI VYSIIFVLGV IANGYVLWVF ARLYPCKKFN EIKIFMVNLT
    61  MADMLFLITL PLWIVYYQNQ GNWILPKFLC NVAGCLFFIN TYCSVAFLGV ITYNRFQAVT
   121  RPIKTAQANT RKRGISLSLV IWVAIVGAAS YFLILDSTNT VPDSAGSGNV TRCFEHYEKG
   181  SVPVLIIHIF IVFSFFLVFL IILFCNLVII RTLLMQPVQQ QRNAEVKRRA LWMVCTVLAV
   241  FIICFVPHHV VQLPWTLAEL GFQDSKFHQA INDAHQVTLC LLSTNCVLDP VIYCFLTKKF
   301  RKHLTEKFYS MRSSRKCSRA TTDTVTEVVV PFNQIPGNSL KN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PTAFR can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

7

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

39 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.85

gnomAD pLI

0.24

gnomAD missense Z

1.45

DepMap mean gene effect

0.03

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• chemotaxis
• G protein-coupled receptor signaling pathway
• immune response
• inflammatory response
• inositol trisphosphate biosynthetic process
• phospholipase C-activating G protein-coupled receptor signaling pathway

Molecular functions

• G protein-coupled purinergic nucleotide receptor activity
• G protein-coupled receptor activity
• lipopolysaccharide binding
• lipopolysaccharide immune receptor activity
• phospholipid binding
• platelet activating factor receptor activity

Cellular components

• membrane
• plasma membrane
• secretory granule membrane
• tertiary granule membrane

Protein domainsUniProt · Pfam · InterPro

• G protein-coupled receptor, rhodopsin-like
• GPCR, rhodopsin-like, 7TM
• 7 transmembrane receptor (rhodopsin family)
• Platelet-activating factor receptor

KeywordsUniProt

• Cell membrane
• Chemotaxis
• Disulfide bond
• G-protein coupled receptor
• Glycoprotein
• Membrane
• Receptor
• Transducer
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of PTAFR in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ARRB1
• MBP
• PRNP