PPAT

Protein identityUniProt · HPA

UniProt accession

Q06203

Gene

PPAT

Ensembl

ENSG00000128059

Chromosome

4

Canonical length

517 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Midbody ring

Quaternary structure

Homotetramer

OverviewNCBI Gene

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]

Canonical amino-acid sequenceUniProt

517 residues, UniProt reviewed canonical sequence.

>Q06203|PPAT
     1  MELEELGIRE ECGVFGCIAS GEWPTQLDVP HVITLGLVGL QHRGQESAGI VTSDGSSVPT
    61  FKSHKGMGLV NHVFTEDNLK KLYVSNLGIG HTRYATTGKC ELENCQPFVV ETLHGKIAVA
   121  HNGELVNAAR LRKKLLRHGI GLSTSSDSEM ITQLLAYTPP QEQDDTPDWV ARIKNLMKEA
   181  PTAYSLLIMH RDVIYAVRDP YGNRPLCIGR LIPVSDINDK EKKTSETEGW VVSSESCSFL
   241  SIGARYYREV LPGEIVEISR HNVQTLDIIS RSEGNPVAFC IFEYVYFARP DSMFEDQMVY
   301  TVRYRCGQQL AIEAPVDADL VSTVPESATP AALAYAGKCG LPYVEVLCKN RYVGRTFIQP
   361  NMRLRQLGVA KKFGVLSDNF KGKRIVLVDD SIVRGNTISP IIKLLKESGA KEVHIRVASP
   421  PIKYPCFMGI NIPTKEELIA NKPEFDHLAE YLGANSVVYL SVEGLVSSVQ EGIKFKKQKE
   481  KKHDIMIQEN GNGLECFEKS GHCTACLTGK YPVELEW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PPAT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.24

Highest tissue expression

8.1 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.3

gnomAD pLI

0.99

gnomAD missense Z

3.06

DepMap mean gene effect

-0.71

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• 'de novo' AMP biosynthetic process
• 'de novo' IMP biosynthetic process
• 'de novo' XMP biosynthetic process
• GMP biosynthetic process
• purine nucleobase biosynthetic process
• purine nucleotide biosynthetic process

Molecular functions

• 4 iron, 4 sulfur cluster binding
• metal ion binding
• amidophosphoribosyltransferase activity

Cellular components

• cytosol

Protein domainsUniProt · Pfam · InterPro

• Phosphoribosyltransferase domain
• Glutamine amidotransferase type 2 domain
• Nucleophile aminohydrolases, N-terminal
• Phosphoribosyltransferase-like
• Phosphoribosyl transferase domain
• Glutamine amidotransferase domain
• Amidophosphoribosyltransferase
• Amidophosphoribosyltransferase, N-terminal

KeywordsUniProt

• 4Fe-4S
• Acetylation
• Allosteric enzyme
• Glutamine amidotransferase
• Glycosyltransferase
• Iron
• Iron-sulfur
• Magnesium
• Metal-binding
• Purine biosynthesis
• Transferase

InteractionsUniProt · HPA

Protein binding partners of PPAT in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NUDT5