PKNOX2

Protein identityUniProt · HPA

UniProt accession

Q96KN3

Gene

PKNOX2

Ensembl

ENSG00000165495

Chromosome

11

Canonical length

472 aa

Protein class

Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Cytokinetic bridge

OverviewNCBI Gene

Homeodomain proteins are sequence-specific transcription factors that share a highly conserved DNA-binding domain and play fundamental roles in cell proliferation, differentiation, and death. PKNOX2 belongs to the TALE (3-amino acid loop extension) class of homeodomain proteins characterized by a 3-amino acid extension between alpha helices 1 and 2 within the homeodomain (Imoto et al., 2001 [PubMed 11549286]).[supplied by OMIM, Oct 2009]

Canonical amino-acid sequenceUniProt

472 residues, UniProt reviewed canonical sequence.

>Q96KN3|PKNOX2
     1  MMQHASPAPA LTMMATQNVP PPPYQDSPQM TATAQPPSKA QAVHISAPSA AASTPVPSAP
    61  IDPQAQLEAD KRAVYRHPLF PLLTLLFEKC EQATQGSECI TSASFDVDIE NFVHQQEQEH
   121  KPFFSDDPEL DNLMVKAIQV LRIHLLELEK VNELCKDFCN RYITCLKTKM HSDNLLRNDL
   181  GGPYSPNQPS INLHSQDLLQ NSPNSMSGVS NNPQGIVVPA SALQQGNIAM TTVNSQVVSG
   241  GALYQPVTMV TSQGQVVTQA IPQGAIQIQN TQVNLDLTSL LDNEDKKSKN KRGVLPKHAT
   301  NIMRSWLFQH LMHPYPTEDE KRQIAAQTNL TLLQVNNWFI NARRRILQPM LDASNPDPAP
   361  KAKKIKSQHR PTQRFWPNSI AAGVLQQQGG APGTNPDGSI NLDNLQSLSS DSATMAMQQA
   421  MMAAHDDSLD GTEEEDEDEM EEEEEEELEE EVDELQTTNV SDLGLEHSDS LE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PKNOX2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.59

Highest tissue expression

34 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.27

gnomAD pLI

0.99

gnomAD missense Z

1.63

DepMap mean gene effect

0.12

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• regulation of transcription by RNA polymerase II

Molecular functions

• actin filament binding
• actin monomer binding
• RNA polymerase II transcription regulatory region sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• actin cytoskeleton
• cytoplasm
• intercellular bridge
• microtubule cytoskeleton
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• Homeodomain
• KN homeodomain
• Homedomain-like superfamily
• Homeobox protein PKNOX/Meis, N-terminal
• Three Amino acid Loop Extension (TALE) homeobox
• Homeobox KN domain
• TALE/MEIS homeobox family N-terminal domain

KeywordsUniProt

• DNA-binding
• Homeobox
• Nucleus

InteractionsUniProt · HPA

Protein binding partners of PKNOX2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PBX4