Seroatlas · Human Serome Atlas

PJA1

E3 ubiquitin-protein ligase Praja-1

Also known as: FLJ11830, PJA1_HUMAN, PRAJA1, RNF70

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NG27
Gene: PJA1
Ensembl: ENSG00000181191
Chromosome: X
Canonical length: 643 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli

OverviewNCBI Gene

This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked cognitive disability disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Canonical amino-acid sequenceUniProt

643 residues, UniProt reviewed canonical sequence.

>Q8NG27|PJA1
     1  MGQESSKPVW PNPTGGYQSN TGRRYGRRHA YVSFRPPTSQ RERIASQRKT NSEVPMHRSA
    61  PSQTTKRSRS PFSTTRRSWD DSESSGTNLN IDNEDYSRYP PREYRASGSR RGMAYGHIDS
   121  YGADDSEEEG AGPVERPPVR GKTGKFKDDK LYDPEKGARS LAGPPPHFSS FSRDVREERD
   181  KLDPVPAARC SASRADFLPQ SSVASQSSSE GKLATKGDSS ERERREQNLP ARPSRAPVSI
   241  CGGGENTSKS AEEPVVRPKI RNLASPNCVK PKIFFDTDDD DDMPHSTSRW RDTANDNEGH
   301  SDGLARRGRG ESSSGYPEPK YPEDKREARS DQVKPEKVPR RRRTMADPDF WTHSDDYYKY
   361  CDEDSDSDKE WIAALRRKYR SREQTLSSSG ESWETLPGKE EREPPQAKVS ASTGTSPGPG
   421  ASASAGAGAG ASAGSNGSNY LEEVREPSLQ EEQASLEEGE IPWLQYHEND SSSEGDNDSG
   481  HELMQPGVFM LDGNNNLEDD SSVSEDLEVD WSLFDGFADG LGVAEAISYV DPQFLTYMAL
   541  EERLAQAMET ALAHLESLAV DVEVANPPAS KESIDALPEI LVTEDHGAVG QEMCCPICCS
   601  EYVKGEVATE LPCHHYFHKP CVSIWLQKSG TCPVCRCMFP PPL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PJA1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.66
Highest tissue expression: 151 nTPM

Expression across tissuesHPA

Tissue

epididymis: 151 nTPM
basal ganglia: 63 nTPM
hypothalamus: 49 nTPM
cerebral cortex: 48 nTPM
amygdala: 43 nTPM
hippocampal formation: 38 nTPM

Single-cell type

epididymal principal cells: 150 nCPM
early spermatids: 106 nCPM
late spermatids: 44 nCPM
migrating cytotrophoblasts: 40 nCPM
cytotrophoblasts: 37 nCPM
basal keratinocytes: 34 nCPM

Immune cell

MAIT T-cell: 34 nTPM
naive CD8 T-cell: 31 nTPM
NK-cell: 30 nTPM
gdT-cell: 30 nTPM
memory CD8 T-cell: 28 nTPM
naive CD4 T-cell: 27 nTPM

Brain region

basal ganglia: 53 nTPM
hypothalamus: 50 nTPM
cerebral cortex: 47 nTPM
white matter: 38 nTPM
hippocampal formation: 37 nTPM
amygdala: 36 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.37
gnomAD pLI: 0.93
gnomAD missense Z: 1.02
DepMap mean gene effect: 0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

cytoplasm

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PJA1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PJA1 as an antibody target. Whether an autoantibody or antibody against PJA1 could matter depends on whether native PJA1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PJA1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PJA1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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