PHF20L1

Protein identityUniProt · HPA

UniProt accession

A8MW92

Gene

PHF20L1

Ensembl

ENSG00000129292

Chromosome

8

Canonical length

1017 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Plasma membrane

OverviewNCBI Gene

Enables methylation-dependent protein binding activity. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process. Predicted to be located in nucleoplasm. Predicted to be part of NSL complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1017 residues, UniProt reviewed canonical sequence.

>A8MW92|PHF20L1
     1  MSKKPPNRPG ITFEIGARLE ALDYLQKWYP SRIEKIDYEE GKMLVHFERW SHRYDEWIYW
    61  DSNRLRPLER PALRKEGLKD EEDFFDFKAG EEVLARWTDC RYYPAKIEAI NKEGTFTVQF
   121  YDGVIRCLKR MHIKAMPEDA KGQVKSQHPL SWCCPIDPAG SCNQSMGSED WIALVKAAAA
   181  AAAKNKTGSK PRTSANSNKD KDKDERKWFK VPSKKEETST CIATPDVEKK EDLPTSSETF
   241  GLHVENVPKM VFPQPESTLS NKRKNNQGNS FQAKRARLNK ITGLLASKAV GVDGAEKKED
   301  YNETAPMLEQ AISPKPQSQK KNEADISSSA NTQKPALLSS TLSSGKARSK KCKHESGDSS
   361  GCIKPPKSPL SPELIQVEDL TLVSQLSSSV INKTSPPQPV NPPRPFKHSE RRRRSQRLAT
   421  LPMPDDSVEK VSSPSPATDG KVFSISSQNQ QESSVPEVPD VAHLPLEKLG PCLPLDLSRG
   481  SEVTAPVASD SSYRNECPRA EKEDTQMLPN PSSKAIADGR GAPAAAGISK TEKKVKLEDK
   541  SSTAFGKRKE KDKERREKRD KDHYRPKQKK KKKKKKKSKQ HDYSDYEDSS LEFLERCSSP
   601  LTRSSGSSLA SRSMFTEKTT TYQYPRAILS VDLSGENLSD VDFLDDSSTE SLLLSGDEYN
   661  QDFDSTNFEE SQDEDDALNE IVRCICEMDE ENGFMIQCEE CLCWQHSVCM GLLEESIPEQ
   721  YICYICRDPP GQRWSAKYRY DKEWLNNGRM CGLSFFKENY SHLNAKKIVS THHLLADVYG
   781  VTEVLHGLQL KIGILKNKHH PDLHLWACSG KRKDQDQIIA GVEKKIAQDT VNREEKKYVQ
   841  NHKEPPRLPL KMEGTYITSE HSYQKPQSFG QDCKSLADPG SSDDDDVSSL EEEQEFHMRS
   901  KNSLQYSAKE HGMPEKNPAE GNTVFVYNDK KGTEDPGDSH LQWQLNLLTH IENVQNEVTS
   961  RMDLIEKEVD VLESWLDFTG ELEPPDPLAR LPQLKRHIKQ LLIDMGKVQQ IATLCSV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PHF20L1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.59

Highest tissue expression

24 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.19

gnomAD pLI

1

gnomAD missense Z

1.85

DepMap mean gene effect

0.04

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 17% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of proteasomal ubiquitin-dependent protein catabolic process
• negative regulation of protein catabolic process
• regulation of transcription by RNA polymerase II

Molecular functions

• methylation-dependent protein binding
• zinc ion binding

Cellular components

• NSL complex
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• Tudor domain
• Zinc finger, FYVE/PHD-type
• Zinc finger, RING/FYVE/PHD-type
• Zinc finger, PHD-type, conserved site
• Lysine-specific demethylase 4-like, Tudor domain
• PHD finger protein 20-like
• Jumonji domain-containing protein 2A Tudor domain
• PhD finger domain
• Agenet domain, plant type
• PHD finger protein 20-like protein 1, Tudor domain
• PHD finger protein 20-like protein 1

KeywordsUniProt

• Acetylation
• Isopeptide bond
• Metal-binding
• Nucleus
• Phosphoprotein
• Repeat
• Ubl conjugation
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of PHF20L1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SOX2
• DNMT1
• KANSL1
• KANSL2