PDK4

Protein identityUniProt · HPA

UniProt accession

Q16654

Gene

PDK4

Ensembl

ENSG00000004799

Chromosome

7

Canonical length

411 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

411 residues, UniProt reviewed canonical sequence.

>Q16654|PDK4
     1  MKAARFVLRS AGSLNGAGLV PREVEHFSRY SPSPLSMKQL LDFGSENACE RTSFAFLRQE
    61  LPVRLANILK EIDILPTQLV NTSSVQLVKS WYIQSLMDLV EFHEKSPDDQ KALSDFVDTL
   121  IKVRNRHHNV VPTMAQGIIE YKDACTVDPV TNQNLQYFLD RFYMNRISTR MLMNQHILIF
   181  SDSQTGNPSH IGSIDPNCDV VAVVQDAFEC SRMLCDQYYL SSPELKLTQV NGKFPDQPIH
   241  IVYVPSHLHH MLFELFKNAM RATVEHQENQ PSLTPIEVIV VLGKEDLTIK ISDRGGGVPL
   301  RIIDRLFSYT YSTAPTPVMD NSRNAPLAGF GYGLPISRLY AKYFQGDLNL YSLSGYGTDA
   361  IIYLKALSSE SIEKLPVFNK SAFKHYQMSS EADDWCIPSR EPKNLAKEVA M

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PDK4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.29

Highest tissue expression

2,155 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.24

gnomAD pLI

0

gnomAD missense Z

0.35

DepMap mean gene effect

0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to fatty acid
• cellular response to starvation
• glucose homeostasis
• insulin receptor signaling pathway
• regulation of bone resorption
• regulation of fatty acid biosynthetic process
• regulation of fatty acid oxidation
• regulation of glucose metabolic process
• regulation of ketone metabolic process
• regulation of pH
• regulation of pyruvate decarboxylation to acetyl-CoA
• response to starvation

Molecular functions

• ATP binding
• protein kinase activity
• pyruvate dehydrogenase (acetyl-transferring) kinase activity

Cellular components

• mitochondrial matrix
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Histidine kinase/HSP90-like ATPase domain
• Histidine kinase domain
• Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase, N-terminal
• Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain superfamily
• Histidine kinase/HSP90-like ATPase superfamily
• PDK/BCKDK protein kinase
• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
• Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase

KeywordsUniProt

• ATP-binding
• Kinase
• Mitochondrion
• Nucleotide-binding
• Transferase
• Transit peptide