PDK2

Protein identityUniProt · HPA

UniProt accession

Q15119

Gene

PDK2

Ensembl

ENSG00000005882

Chromosome

17

Canonical length

407 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Mitochondria

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Canonical amino-acid sequenceUniProt

407 residues, UniProt reviewed canonical sequence.

>Q15119|PDK2
     1  MRWVWALLKN ASLAGAPKYI EHFSKFSPSP LSMKQFLDFG SSNACEKTSF TFLRQELPVR
    61  LANIMKEINL LPDRVLSTPS VQLVQSWYVQ SLLDIMEFLD KDPEDHRTLS QFTDALVTIR
   121  NRHNDVVPTM AQGVLEYKDT YGDDPVSNQN IQYFLDRFYL SRISIRMLIN QHTLIFDGST
   181  NPAHPKHIGS IDPNCNVSEV VKDAYDMAKL LCDKYYMASP DLEIQEINAA NSKQPIHMVY
   241  VPSHLYHMLF ELFKNAMRAT VESHESSLIL PPIKVMVALG EEDLSIKMSD RGGGVPLRKI
   301  ERLFSYMYST APTPQPGTGG TPLAGFGYGL PISRLYAKYF QGDLQLFSME GFGTDAVIYL
   361  KALSTDSVER LPVYNKSAWR HYQTIQEAGD WCVPSTEPKN TSTYRVS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PDK2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.28

Highest tissue expression

266 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.92

gnomAD pLI

0

gnomAD missense Z

2.05

DepMap mean gene effect

-0.25

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to nutrient
• cellular response to reactive oxygen species
• glucose homeostasis
• glucose metabolic process
• insulin receptor signaling pathway
• intrinsic apoptotic signaling pathway by p53 class mediator
• regulation of calcium-mediated signaling
• regulation of gluconeogenesis
• regulation of glucose metabolic process
• regulation of ketone metabolic process
• regulation of pH
• regulation of pyruvate decarboxylation to acetyl-CoA

Molecular functions

• ATP binding
• protein homodimerization activity
• protein kinase activity
• pyruvate dehydrogenase (acetyl-transferring) kinase activity

Cellular components

• cytosol
• mitochondrial matrix
• mitochondrion
• nucleoplasm
• pyruvate dehydrogenase complex

Protein domainsUniProt · Pfam · InterPro

• Histidine kinase/HSP90-like ATPase domain
• Histidine kinase domain
• Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase, N-terminal
• Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain superfamily
• Histidine kinase/HSP90-like ATPase superfamily
• PDK/BCKDK protein kinase
• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
• Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase

KeywordsUniProt

• ATP-binding
• Kinase
• Mitochondrion
• Nucleotide-binding
• Phosphoprotein
• Transferase
• Transit peptide

InteractionsUniProt · HPA

Protein binding partners of PDK2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DLAT