PADI4

Protein identityUniProt · HPA

UniProt accession

Q9UM07

Gene

PADI4

Ensembl

ENSG00000159339

Chromosome

1

Canonical length

663 aa

Protein class

Disease related genes, Enzymes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins

OverviewNCBI Gene

This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

663 residues, UniProt reviewed canonical sequence.

>Q9UM07|PADI4
     1  MAQGTLIRVT PEQPTHAVCV LGTLTQLDIC SSAPEDCTSF SINASPGVVV DIAHGPPAKK
    61  KSTGSSTWPL DPGVEVTLTM KVASGSTGDQ KVQISYYGPK TPPVKALLYL TGVEISLCAD
   121  ITRTGKVKPT RAVKDQRTWT WGPCGQGAIL LVNCDRDNLE SSAMDCEDDE VLDSEDLQDM
   181  SLMTLSTKTP KDFFTNHTLV LHVARSEMDK VRVFQATRGK LSSKCSVVLG PKWPSHYLMV
   241  PGGKHNMDFY VEALAFPDTD FPGLITLTIS LLDTSNLELP EAVVFQDSVV FRVAPWIMTP
   301  NTQPPQEVYA CSIFENEDFL KSVTTLAMKA KCKLTICPEE ENMDDQWMQD EMEIGYIQAP
   361  HKTLPVVFDS PRNRGLKEFP IKRVMGPDFG YVTRGPQTGG ISGLDSFGNL EVSPPVTVRG
   421  KEYPLGRILF GDSCYPSNDS RQMHQALQDF LSAQQVQAPV KLYSDWLSVG HVDEFLSFVP
   481  APDRKGFRLL LASPRSCYKL FQEQQNEGHG EALLFEGIKK KKQQKIKNIL SNKTLREHNS
   541  FVERCIDWNR ELLKRELGLA ESDIIDIPQL FKLKEFSKAE AFFPNMVNML VLGKHLGIPK
   601  PFGPVINGRC CLEEKVCSLL EPLGLQCTFI NDFFTYHIRH GEVHCGTNVR RKPFSFKWWN
   661  MVP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PADI4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

99 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PADI4.

Disease | AllUniProt

Conditions PADI4 is implicated in, by any mechanism.

• Rheumatoid arthritis (RA) MIM:180300

Disease | ImmuneIEDB

Conditions an epitope on PADI4 was assayed in.

• rheumatoid arthritis B cell

ReferencesPubMed · IEDB

Publications for PADI4 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.14

gnomAD pLI

0

gnomAD missense Z

-0.08

DepMap mean gene effect

0.03

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• chromatin organization
• chromatin remodeling
• innate immune response
• nucleosome assembly
• post-translational protein modification
• protein modification process
• stem cell population maintenance

Molecular functions

• calcium ion binding
• histone arginine deiminase activity
• histone H3R26 arginine deiminase activity
• identical protein binding
• protein-arginine deiminase activity
• histone H3R17 arginine deiminase activity
• histone H3R2 arginine deiminase activity
• histone H3R8 arginine deiminase activity

Cellular components

• cytoplasm
• cytosol
• nucleoplasm
• nucleus
• protein-containing complex

Protein domainsUniProt · Pfam · InterPro

• Protein-arginine deiminase
• Cupredoxin
• Protein-arginine deiminase, C-terminal
• Protein-arginine deiminase (PAD), N-terminal
• Protein-arginine deiminase (PAD), central domain
• Protein-arginine deiminase, central domain superfamily
• PAD, N-terminal domain superfamily
• Protein-arginine deiminase (PAD)
• Protein-arginine deiminase (PAD) N-terminal domain
• Protein-arginine deiminase (PAD) middle domain

KeywordsUniProt

• Calcium
• Chromatin regulator
• Citrullination
• Cytoplasm
• Hydrolase
• Immunity
• Innate immunity
• Metal-binding
• Nucleus
• Transcription
• Transcription regulation