NTN3

Protein identityUniProt · HPA

UniProt accession

O00634

Gene

NTN3

Ensembl

ENSG00000162068

Chromosome

16

Canonical length

580 aa

Protein class

Predicted secreted proteins

Secretome location

Secreted in brain

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in Golgi apparatus and extracellular region. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

580 residues, UniProt reviewed canonical sequence.

>O00634|NTN3
     1  MPGWPWGLLL TAGTLFAALS PGPPAPADPC HDEGGAPRGC VPGLVNAALG REVLASSTCG
    61  RPATRACDAS DPRRAHSPAL LTSPGGTASP LCWRSESLPR APLNVTLTVP LGKAFELVFV
   121  SLRFCSAPPA SVALLKSQDH GRSWAPLGFF SSHCDLDYGR LPAPANGPAG PGPEALCFPA
   181  PLAQPDGSGL LAFSMQDSSP PGLDLDSSPV LQDWVTATDV RVVLTRPSTA GDPRDMEAVV
   241  PYSYAATDLQ VGGRCKCNGH ASRCLLDTQG HLICDCRHGT EGPDCGRCKP FYCDRPWQRA
   301  TARESHACLA CSCNGHARRC RFNMELYRLS GRRSGGVCLN CRHNTAGRHC HYCREGFYRD
   361  PGRALSDRRA CRACDCHPVG AAGKTCNQTT GQCPCKDGVT GLTCNRCAPG FQQSRSPVAP
   421  CVKTPIPGPT EDSSPVQPQD CDSHCKPARG SYRISLKKFC KKDYAVQVAV GARGEARGAW
   481  TRFPVAVLAV FRSGEERARR GSSALWVPAG DAACGCPRLL PGRRYLLLGG GPGAAAGGAG
   541  GRGPGLIAAR GSLVLPWRDA WTRRLRRLQR RERRGRCSAA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NTN3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Secreted

Secreted

Yes

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

0.3 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.21

gnomAD pLI

0

gnomAD missense Z

0.28

DepMap mean gene effect

0.14

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• axon guidance
• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription factor activity, RNA polymerase II-specific
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• signaling receptor binding

Cellular components

• extracellular region
• Golgi apparatus

Protein domainsUniProt · Pfam · InterPro

• Netrin domain
• Laminin-type EGF domain
• Laminin, N-terminal
• Tissue inhibitor of metalloproteinases-like, OB-fold
• Netrin module, non-TIMP type
• Laminin/Netrin Extracellular Matrix
• Laminin/attractin/netrin-like, EGF domain
• Laminin EGF domain
• Laminin N-terminal (Domain VI)
• UNC-6/NTR/C345C module
• Laminin/attractin EGF domain

KeywordsUniProt

• Disulfide bond
• Extracellular matrix
• Glycoprotein
• Laminin EGF-like domain
• Repeat
• Secreted
• Signal

InteractionsUniProt · HPA

Protein binding partners of NTN3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CDON
• BOC