Seroatlas · Human Serome Atlas

NSUN4

5-cytosine rRNA methyltransferase NSUN4

Also known as: MGC22960, NSUN4_HUMAN, SHTAP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96CB9
Gene
NSUN4
Ensembl
ENSG00000117481
Chromosome
1
Canonical length
384 aa
Protein class
Predicted intracellular proteins

OverviewNCBI Gene

Enables mRNA (cytidine-5-)-methyltransferase activity and rRNA (cytosine-C5-)-methyltransferase activity. Involved in mitochondrial RNA catabolic process; mitochondrial RNA modification; and rRNA methylation. Located in mitochondrial matrix. Part of mitochondrial large ribosomal subunit. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

384 residues, UniProt reviewed canonical sequence.

>Q96CB9|NSUN4
     1  MAALTLRGVR ELLKRVDLAT VPRRHRYKKK WAATEPKFPA VRLALQNFDM TYSVQFGDLW
    61  PSIRVSLLSE QKYGALVNNF AAWDHVSAKL EQLSAKDFVN EAISHWELQS EGGQSAAPSP
   121  ASWACSPNLR CFTFDRGDIS RFPPARPGSL GVMEYYLMDA ASLLPVLALG LQPGDIVLDL
   181  CAAPGGKTLA LLQTGCCRNL AANDLSPSRI ARLQKILHSY VPEEIRDGNQ VRVTSWDGRK
   241  WGELEGDTYD RVLVDVPCTT DRHSLHEEEN NIFKRSRKKE RQILPVLQVQ LLAAGLLATK
   301  PGGHVVYSTC SLSHLQNEYV VQGAIELLAN QYSIQVQVED LTHFRRVFMD TFCFFSSCQV
   361  GELVIPNLMA NFGPMYFCKM RRLT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NSUN4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.28
Highest tissue expression
80 nTPM

Expression across tissuesHPA

Tissue

  • testis: 80 nTPM
  • parathyroid gland: 7.6 nTPM
  • tongue: 6.5 nTPM
  • skeletal muscle: 6.4 nTPM
  • thyroid gland: 6.3 nTPM
  • liver: 6.2 nTPM

Single-cell type

  • late spermatids: 922 nCPM
  • early spermatids: 167 nCPM
  • epicardial cells: 71 nCPM
  • neutrophils: 61 nCPM
  • late primary spermatocytes: 50 nCPM
  • proximal tubule cells: 43 nCPM

Immune cell

  • memory B-cell: 5 nTPM
  • myeloid DC: 4.9 nTPM
  • plasmacytoid DC: 4.4 nTPM
  • classical monocyte: 4.1 nTPM
  • eosinophil: 4 nTPM
  • naive CD4 T-cell: 3.6 nTPM

Brain region

  • pons: 15 nTPM
  • white matter: 14 nTPM
  • choroid plexus: 14 nTPM
  • hypothalamus: 13 nTPM
  • midbrain: 13 nTPM
  • thalamus: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.91
gnomAD pLI
0
gnomAD missense Z
0.83
DepMap mean gene effect
-0.39
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NSUN4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NSUN4 as an antibody target. Whether an autoantibody or antibody against NSUN4 could matter depends on whether native NSUN4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NSUN4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NSUN4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NSUN4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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