NOX1

Protein identityUniProt · HPA

UniProt accession

Q9Y5S8

Gene

NOX1

Ensembl

ENSG00000007952

Chromosome

X

Canonical length

564 aa

Protein class

Disease related genes, Plasma proteins, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins, Transporters

OverviewNCBI Gene

This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

Canonical amino-acid sequenceUniProt

564 residues, UniProt reviewed canonical sequence.

>Q9Y5S8|NOX1
     1  MGNWVVNHWF SVLFLVVWLG LNVFLFVDAF LKYEKADKYY YTRKILGSTL ACARASALCL
    61  NFNSTLILLP VCRNLLSFLR GTCSFCSRTL RKQLDHNLTF HKLVAYMICL HTAIHIIAHL
   121  FNFDCYSRSR QATDGSLASI LSSLSHDEKK GGSWLNPIQS RNTTVEYVTF TSIAGLTGVI
   181  MTIALILMVT SATEFIRRSY FEVFWYTHHL FIFYILGLGI HGIGGIVRGQ TEESMNESHP
   241  RKCAESFEMW DDRDSHCRRP KFEGHPPESW KWILAPVILY ICERILRFYR SQQKVVITKV
   301  VMHPSKVLEL QMNKRGFSME VGQYIFVNCP SISLLEWHPF TLTSAPEEDF FSIHIRAAGD
   361  WTENLIRAFE QQYSPIPRIE VDGPFGTASE DVFQYEVAVL VGAGIGVTPF ASILKSIWYK
   421  FQCADHNLKT KKIYFYWICR ETGAFSWFNN LLTSLEQEME ELGKVGFLNY RLFLTGWDSN
   481  IVGHAALNFD KATDIVTGLK QKTSFGRPMW DNEFSTIATS HPKSVVGVFL CGPRTLAKSL
   541  RKCCHRYSSL DPRKVQFYFN KENF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NOX1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

6

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

67 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.06

gnomAD pLI

0

gnomAD missense Z

-0.05

DepMap mean gene effect

-0.12

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• angiogenesis
• cell migration
• cellular response to hyperoxia
• defense response
• extracellular matrix organization
• hydrogen peroxide metabolic process
• inflammatory response
• intrinsic apoptotic signaling pathway in response to oxidative stress
• JNK cascade
• NADP+ metabolic process
• oxygen metabolic process
• positive regulation of cell population proliferation
• positive regulation of integrin biosynthetic process
• positive regulation of JNK cascade
• positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
• positive regulation of smooth muscle cell proliferation
• positive regulation of vascular endothelial growth factor production
• regulation of blood pressure
• regulation of systemic arterial blood pressure by renin-angiotensin
• respiratory burst
• signal transduction
• superoxide anion generation

Molecular functions

• metal ion binding
• NAD(P)H oxidase H2O2-forming activity
• NADP binding
• small GTPase binding
• superoxide-generating NAD(P)H oxidase activity
• superoxide-generating NADPH oxidase activity

Cellular components

• cell projection
• early endosome
• NADPH oxidase complex
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Cytochrome b245, heavy chain
• FAD-binding 8
• Ferric reductase, NAD binding domain
• Ferric reductase transmembrane component-like domain
• FAD-binding domain, ferredoxin reductase-type
• Riboflavin synthase-like beta-barrel
• Ferredoxin-NADP reductase (FNR), nucleotide-binding domain
• Respiratory burst oxidase/Ferric reductase
• Ferric reductase like transmembrane component
• FAD-binding domain
• Ferric reductase NAD binding domain

KeywordsUniProt

• Cell junction
• Cell membrane
• Cell projection
• Electron transport
• FAD
• Flavoprotein
• Glycoprotein
• Heme
• Iron
• Membrane
• Metal-binding
• NADP
• Oxidoreductase
• Phosphoprotein
• Transmembrane
• Transmembrane helix
• Transport

InteractionsUniProt · HPA

Protein binding partners of NOX1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NOXO1
• CYBA
• NOXA1