NOL3
Nucleolar protein 3
Also known as: ARC, CARD2, MYP, NOL3_HUMAN, NOP30
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- O60936
- Gene
- NOL3
- Ensembl
- ENSG00000140939
- Chromosome
- 16
- Canonical length
- 208 aa
- Protein class
- Disease related genes, Predicted intracellular proteins
- Subcellular location
- Nucleoplasm,Cytosol
OverviewNCBI Gene
This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Canonical amino-acid sequenceUniProt
208 residues, UniProt reviewed canonical sequence.
>O60936|NOL3
1 MGNAQERPSE TIDRERKRLV ETLQADSGLL LDALLARGVL TGPEYEALDA LPDAERRVRR
61 LLLLVQGKGE AACQELLRCA QRTAGAPDPA WDWQHVGPGY RDRSYDPPCP GHWTPEAPGS
121 GTTCPGLPRA SDPDEAGGPE GSEAVQSGTP EEPEPELEAE ASKEAEPEPE PEPELEPEAE
181 AEPEPELEPE PDPEPEPDFE ERDESEDSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NOL3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.55
- Highest tissue expression
- 134 nTPM
Expression across tissuesHPA
Tissue
- skeletal muscle: 134 nTPM
- heart muscle: 93 nTPM
- tongue: 91 nTPM
- skin: 76 nTPM
- adrenal gland: 75 nTPM
- prostate: 68 nTPM
Single-cell type
- esophageal apical cells: 329 nCPM
- myonuclei: 115 nCPM
- esophageal suprabasal cells: 109 nCPM
- thymic myoid cells: 98 nCPM
- vascular smooth muscle cells: 84 nCPM
- breast lactating cells: 79 nCPM
Immune cell
- classical monocyte: 3.4 nTPM
- memory CD4 T-cell: 1.7 nTPM
- total PBMC: 1.3 nTPM
- myeloid DC: 1 nTPM
- plasmacytoid DC: 0.9 nTPM
- neutrophil: 0.8 nTPM
Brain region
- medulla oblongata: 47 nTPM
- pons: 37 nTPM
- white matter: 34 nTPM
- thalamus: 33 nTPM
- basal ganglia: 32 nTPM
- cerebellum: 32 nTPM
DiseaseUniProt · ClinVar · IEDB · PubMed
Four sources answering four different questions about NOL3.
Disease | AllUniProt
Conditions NOL3 is implicated in, by any mechanism.
- Myoclonus, familial, 1 (MYOCL1) MIM:614937
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.62
- gnomAD pLI
- 0
- gnomAD missense Z
- -0.13
- DepMap mean gene effect
- -0.15
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- blood vessel remodeling
- cardiac muscle cell apoptotic process
- intrinsic apoptotic signaling pathway
- mRNA splice site recognition
- negative regulation of apoptotic process
- negative regulation of cardiac muscle cell apoptotic process
- negative regulation of extrinsic apoptotic signaling pathway
- negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
- negative regulation of intrinsic apoptotic signaling pathway
- negative regulation of mitochondrial membrane permeability involved in apoptotic process
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
- negative regulation of programmed necrotic cell death
- negative regulation of release of cytochrome c from mitochondria
- negative regulation of tumor necrosis factor-mediated signaling pathway
- protein complex oligomerization
- regulation of gene expression
- regulation of non-canonical NF-kappaB signal transduction
- release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
- response to hypoxia
- response to ischemia
- RNA splicing
- negative regulation of muscle atrophy
- response to injury involved in regulation of muscle adaptation
Molecular functions
- calcium ion binding
- caspase binding
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process
- death effector domain binding
- death receptor binding
- identical protein binding
- RNA binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of NOL3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NOL3 as an antibody target. Whether an autoantibody or antibody against NOL3 could matter depends on whether native NOL3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NOL3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NOL3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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