NME5

Protein identityUniProt · HPA

UniProt accession

P56597

Gene

NME5

Ensembl

ENSG00000112981

Chromosome

5

Canonical length

212 aa

Protein class

Human disease related genes, Predicted intracellular proteins

Subcellular location

Plasma membrane,Primary cilium,Cytosol

OverviewNCBI Gene

Enables 3'-5' exonuclease activity. Involved in spermatid development. Predicted to be located in 9+2 motile cilium. Predicted to be part of radial spoke. Predicted to be active in cilium. Implicated in primary ciliary dyskinesia. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

212 residues, UniProt reviewed canonical sequence.

>P56597|NME5
     1  MEISMPPPQI YVEKTLAIIK PDIVDKEEEI QDIILRSGFT IVQRRKLRLS PEQCSNFYVE
    61  KYGKMFFPNL TAYMSSGPLV AMILARHKAI SYWLELLGPN NSLVAKETHP DSLRAIYGTD
   121  DLRNALHGSN DFAAAEREIR FMFPEVIVEP IPIGQAAKDY LNLHIMPTLL EGLTELCKQK
   181  PADPLIWLAD WLLKNNPNKP KLCHHPIVEE PY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NME5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.36

Highest tissue expression

50 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NME5.

Disease | AllUniProt

Conditions NME5 is implicated in, by any mechanism.

• Ciliary dyskinesia, primary, 48, without situs inversus (CILD48) MIM:620032

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 36 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Ciliary dyskinesia, primary, 48, without situs inversus

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.28

gnomAD pLI

0

gnomAD missense Z

0.41

DepMap mean gene effect

-0.12

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cilium assembly
• cilium movement
• CTP biosynthetic process
• DNA catabolic process
• epithelial cilium movement involved in extracellular fluid movement
• establishment of localization in cell
• GTP biosynthetic process
• negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
• nucleoside metabolic process
• spermatid development
• spermatogenesis
• UTP biosynthetic process
• ventricular system development

Molecular functions

• 3'-5'-DNA exonuclease activity

Cellular components

• 9+2 motile cilium
• cilium
• extracellular region
• radial spoke
• sperm flagellum

Protein domainsUniProt · Pfam · InterPro

• Nucleoside diphosphate kinase
• Dpy-30 motif
• Nucleoside diphosphate kinase-like domain
• Nucleoside diphosphate kinase-like domain superfamily
• Nucleoside diphosphate kinase
• Dpy-30 motif
• Nucleoside-diphosphate kinase-like NDK-H5

KeywordsUniProt

• Cell projection
• Ciliopathy
• Cilium
• Cytoplasm
• Cytoskeleton
• Developmental protein
• Differentiation
• Flagellum
• Hydrolase
• Primary ciliary dyskinesia
• Spermatogenesis

InteractionsUniProt · HPA

Protein binding partners of NME5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• IQUB
• DYDC2