NLRP4

Protein identityUniProt · HPA

UniProt accession

Q96MN2

Gene

NLRP4

Ensembl

ENSG00000160505

Chromosome

19

Canonical length

994 aa

Protein class

Predicted intracellular proteins

Subcellular location

Mitochondria

OverviewNCBI Gene

The protein encoded by this gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). This gene product has a demonstrated role as a negative regulator of autophagy and type I interferon signaling pathways as a result of protein interactions with its NACHT domain. The PYD domain has also been shown to be important in the inhibition of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells). [provided by RefSeq, Dec 2016]

Canonical amino-acid sequenceUniProt

994 residues, UniProt reviewed canonical sequence.

>Q96MN2|NLRP4
     1  MAASFFSDFG LMWYLEELKK EEFRKFKEHL KQMTLQLELK QIPWTEVKKA SREELANLLI
    61  KHYEEQQAWN ITLRIFQKMD RKDLCMKVMR ERTGYTKTYQ AHAKQKFSRL WSSKSVTEIH
   121  LYFEEEVKQE ECDHLDRLFA PKEAGKQPRT VIIQGPQGIG KTTLLMKLMM AWSDNKIFRD
   181  RFLYTFYFCC RELRELPPTS LADLISREWP DPAAPITEIV SQPERLLFVI DSFEELQGGL
   241  NEPDSDLCGD LMEKRPVQVL LSSLLRKKML PEASLLIAIK PVCPKELRDQ VTISEIYQPR
   301  GFNESDRLVY FCCFFKDPKR AMEAFNLVRE SEQLFSICQI PLLCWILCTS LKQEMQKGKD
   361  LALTCQSTTS VYSSFVFNLF TPEGAEGPTP QTQHQLKALC SLAAEGMWTD TFEFCEDDLR
   421  RNGVVDADIP ALLGTKILLK YGERESSYVF LHVCIQEFCA ALFYLLKSHL DHPHPAVRCV
   481  QELLVANFEK ARRAHWIFLG CFLTGLLNKK EQEKLDAFFG FQLSQEIKQQ IHQCLKSLGE
   541  RGNPQGQVDS LAIFYCLFEM QDPAFVKQAV NLLQEANFHI IDNVDLVVSA YCLKYCSSLR
   601  KLCFSVQNVF KKEDEHSSTS DYSLICWHHI CSVLTTSGHL RELQVQDSTL SESTFVTWCN
   661  QLRHPSCRLQ KLGINNVSFS GQSVLLFEVL FYQPDLKYLS FTLTKLSRDD IRSLCDALNY
   721  PAGNVKELAL VNCHLSPIDC EVLAGLLTNN KKLTYLNVSC NQLDTGVPLL CEALCSPDTV
   781  LVYLMLAFCH LSEQCCEYIS EMLLRNKSVR YLDLSANVLK DEGLKTLCEA LKHPDCCLDS
   841  LCLVKCFITA AGCEDLASAL ISNQNLKILQ IGCNEIGDVG VQLLCRALTH TDCRLEILGL
   901  EECGLTSTCC KDLASVLTCS KTLQQLNLTL NTLDHTGVVV LCEALRHPEC ALQVLGLRKT
   961  DFDEETQALL TAEEERNPNL TITDDCDTIT RVEI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NLRP4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

6.6 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.78

gnomAD pLI

0

gnomAD missense Z

-0.74

DepMap mean gene effect

0.02

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• inflammatory response
• negative regulation of innate immune response
• regulation of inflammatory response

Molecular functions

• ATP binding
• molecular adaptor activity

Cellular components

• cytoplasm
• cytosol

Protein domainsUniProt · Pfam · InterPro

• Leucine-rich repeat
• DAPIN domain
• NACHT nucleoside triphosphatase
• Death-like domain superfamily
• P-loop containing nucleoside triphosphate hydrolase
• Leucine-rich repeat domain superfamily
• NOD1/2, winged helix domain
• NACHT, LRR and PYD domains-containing protein, helical domain HD2
• NLRP family, innate immunity and inflammation regulators
• PAAD/DAPIN/Pyrin domain
• NACHT domain
• Leucine Rich repeat
• NLRC4 helical domain HD2
• NOD2 winged helix domain

KeywordsUniProt

• ATP-binding
• Inflammatory response
• Leucine-rich repeat
• Nucleotide-binding
• Repeat

InteractionsUniProt · HPA

Protein binding partners of NLRP4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DTX4