Seroatlas · Human Serome Atlas

NIF3L1

NIF3-like protein 1

Also known as: ALS2CR1, CALS-7, MDS015, NIF3L_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9GZT8
Gene
NIF3L1
Ensembl
ENSG00000196290
Chromosome
2
Canonical length
377 aa
Protein class
Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Vesicles
Quaternary structure
Homodimer

OverviewNCBI Gene

Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and identical protein binding activity. Involved in positive regulation of DNA-templated transcription. Located in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

377 residues, UniProt reviewed canonical sequence.

>Q9GZT8|NIF3L1
     1  MLSSCVRPVP TTVRFVDSLI CNSSRSFMDL KALLSSLNDF ASLSFAESWD NVGLLVEPSP
    61  PHTVNTLFLT NDLTEEVMEE VLQKKADLIL SYHPPIFRPM KRITWNTWKE RLVIRALENR
   121  VGIYSPHTAY DAAPQGVNNW LAKGLGACTS RPIHPSKAPN YPTEGNHRVE FNVNYTQDLD
   181  KVMSAVKGID GVSVTSFSAR TGNEEQTRIN LNCTQKALMQ VVDFLSRNKQ LYQKTEILSL
   241  EKPLLLHTGM GRLCTLDESV SLATMIDRIK RHLKLSHIRL ALGVGRTLES QVKVVALCAG
   301  SGSSVLQGVE ADLYLTGEMS HHDTLDAASQ GINVILCEHS NTERGFLSDL RDMLDSHLEN
   361  KINIILSETD RDPLQVV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NIF3L1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
30 nTPM

Expression across tissuesHPA

Tissue

  • tonsil: 30 nTPM
  • retina: 30 nTPM
  • tongue: 29 nTPM
  • rectum: 26 nTPM
  • parathyroid gland: 25 nTPM
  • epididymis: 25 nTPM

Single-cell type

  • oocytes: 106 nCPM
  • retinal bipolar cells: 69 nCPM
  • extravillous trophoblasts: 49 nCPM
  • cytotrophoblasts: 48 nCPM
  • migrating cytotrophoblasts: 46 nCPM
  • differentiating spermatogonia: 43 nCPM

Immune cell

  • basophil: 71 nTPM
  • naive CD4 T-cell: 36 nTPM
  • memory CD8 T-cell: 35 nTPM
  • intermediate monocyte: 35 nTPM
  • NK-cell: 34 nTPM
  • T-reg: 32 nTPM

Brain region

  • white matter: 24 nTPM
  • cerebral cortex: 22 nTPM
  • pons: 22 nTPM
  • basal ganglia: 21 nTPM
  • cerebellum: 20 nTPM
  • spinal cord: 20 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NIF3L1.

Disease | ImmuneIEDB

Conditions an epitope on NIF3L1 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.28
gnomAD pLI
0
gnomAD missense Z
-0.22
DepMap mean gene effect
-0.06
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • DUF34/NIF3
  • DUF34/NIF3, animal
  • DUF34/NIF3 superfamily
  • Duf34/NIF3 (NGG1p interacting factor 3)

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NIF3L1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NIF3L1 as an antibody target. Whether an autoantibody or antibody against NIF3L1 could matter depends on whether native NIF3L1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NIF3L1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NIF3L1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NIF3L1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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