Seroatlas · Human Serome Atlas

NGEF

Ephexin-1

Also known as: ARHGEF27, NGEF_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N5V2
Gene
NGEF
Ensembl
ENSG00000066248
Chromosome
2
Canonical length
710 aa
Protein class
Predicted intracellular proteins
Subcellular location
Plasma membrane,Cytosol

OverviewNCBI Gene

Enables guanyl-nucleotide exchange factor activity. Predicted to be involved in ephrin receptor signaling pathway; regulation of actin cytoskeleton organization; and regulation of synapse organization. Predicted to be located in cytosol. Predicted to be active in glutamatergic synapse and postsynapse. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

710 residues, UniProt reviewed canonical sequence.

>Q8N5V2|NGEF
     1  METRESEDLE KTRRKSASDQ WNTDNEPAKV KPELLPEKEE TSQADQDIQD KEPHCHIPIK
    61  RNSIFNRSIR RKSKAKARDN PERNASCLAD SQDNGKSVNE PLTLNIPWSR MPPCRTAMQT
   121  DPGAQEMSES SSTPGNGATP EEWPALADSP TTLTEALRMI HPIPADSWRN LIEQIGLLYQ
   181  EYRDKSTLQE IETRRQQDAE IEDNTNGSPA SEDTPEEEEE EEEEEEPASP PERKTLPQIC
   241  LLSNPHSRFN LWQDLPEIRS SGVLEILQPE EIKLQEAMFE LVTSEASYYK SLNLLVSHFM
   301  ENERIRKILH PSEAHILFSN VLDVLAVSER FLLELEHRME ENIVISDVCD IVYRYAADHF
   361  SVYITYVSNQ TYQERTYKQL LQEKAAFREL IAQLELDPKC RGLPFSSFLI LPFQRITRLK
   421  LLVQNILKRV EERSERECTA LDAHKELEMV VKACNEGVRK MSRTEQMISI QKKMEFKIKS
   481  VPIISHSRWL LKQGELQQMS GPKTSRTLRT KKLFHEIYLF LFNDLLVICR QIPGDKYQVF
   541  DSAPRGLLRV EELEDQGQTL ANVFILRLLE NADDREATYM LKASSQSEMK RWMTSLAPNR
   601  RTKFVSFTSR LLDCPQVQCV HPYVAQQPDE LTLELADILN ILDKTDDGWI FGERLHDQER
   661  GWFPSSMTEE ILNPKIRSQN LKECFRVHKM DDPQRSQNKD RRKLGSRNRQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NGEF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.39
Highest tissue expression
140 nTPM

Expression across tissuesHPA

Tissue

  • basal ganglia: 140 nTPM
  • cerebral cortex: 139 nTPM
  • adrenal gland: 54 nTPM
  • duodenum: 40 nTPM
  • liver: 36 nTPM
  • small intestine: 29 nTPM

Single-cell type

  • enterocytes: 135 nCPM
  • brain inhibitory neurons: 123 nCPM
  • colonocytes: 69 nCPM
  • proximal tubule cells: 56 nCPM
  • brain excitatory neurons: 51 nCPM
  • hepatocytes: 51 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • basal ganglia: 380 nTPM
  • cerebral cortex: 234 nTPM
  • white matter: 166 nTPM
  • hippocampal formation: 64 nTPM
  • amygdala: 61 nTPM
  • thalamus: 57 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.39
gnomAD pLI
0.66
gnomAD missense Z
1.66
DepMap mean gene effect
0.04
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NGEF in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NGEF as an antibody target. Whether an autoantibody or antibody against NGEF could matter depends on whether native NGEF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NGEF is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NGEF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NGEF. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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