Seroatlas · Human Serome Atlas

NETO1

Neuropilin and tolloid-like protein 1

Also known as: BCTL1, BTCL1, NETO1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8TDF5
Gene
NETO1
Ensembl
ENSG00000166342
Chromosome
18
Canonical length
533 aa
Protein class
Predicted intracellular proteins, Predicted membrane proteins, Predicted secreted proteins, Transporters
Subcellular location
Golgi apparatus
Secretome location
Secreted in brain

OverviewNCBI Gene

This gene encodes a transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. This protein is thought to play a critical role in spatial learning and memory by regulating the function of synaptic N-methyl-D-aspartic acid receptor complexes in the hippocampus. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2017]

Canonical amino-acid sequenceUniProt

533 residues, UniProt reviewed canonical sequence.

>Q8TDF5|NETO1
     1  MIHGRSVLHI VASLIILHLS GATKKGTEKQ TTSETQKSVQ CGTWTKHAEG GIFTSPNYPS
    61  KYPPDRECIY IIEAAPRQCI ELYFDEKYSI EPSWECKFDH IEVRDGPFGF SPIIGRFCGQ
   121  QNPPVIKSSG RFLWIKFFAD GELESMGFSA RYNFTPDPDF KDLGALKPLP ACEFEMGGSE
   181  GIVESIQIMK EGKATASEAV DCKWYIRAPP RSKIYLRFLD YEMQNSNECK RNFVAVYDGS
   241  SSVEDLKAKF CSTVANDVML RTGLGVIRMW ADEGSRNSRF QMLFTSFQEP PCEGNTFFCH
   301  SNMCINNTLV CNGLQNCVYP WDENHCKEKR KTSLLDQLTN TSGTVIGVTS CIVIILIIIS
   361  VIVQIKQPRK KYVQRKSDFD QTVFQEVFEP PHYELCTLRG TGATADFADV ADDFENYHKL
   421  RRSSSKCIHD HHCGSQLSST KGSRSNLSTR DASILTEMPT QPGKPLIPPM NRRNILVMKH
   481  SYSQDAADAC DIDEIEEVPT TSHRLSRHDK AVQRFCLIGS LSKHESEYNT TRV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NETO1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.46
Highest tissue expression
32 nTPM

Expression across tissuesHPA

Tissue

  • retina: 32 nTPM
  • cerebral cortex: 25 nTPM
  • basal ganglia: 12 nTPM
  • hippocampal formation: 11 nTPM
  • amygdala: 6.9 nTPM
  • hypothalamus: 3.6 nTPM

Single-cell type

  • retinal bipolar cells: 1,084 nCPM
  • brain inhibitory neurons: 333 nCPM
  • brain excitatory neurons: 195 nCPM
  • oligodendrocyte progenitor cells: 191 nCPM
  • b-cells: 92 nCPM
  • other brain neurons: 60 nCPM

Immune cell

  • naive B-cell: 0.8 nTPM
  • memory B-cell: 0.5 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM

Brain region

  • cerebral cortex: 78 nTPM
  • white matter: 65 nTPM
  • hippocampal formation: 60 nTPM
  • basal ganglia: 43 nTPM
  • amygdala: 29 nTPM
  • pons: 25 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.5
gnomAD pLI
0.05
gnomAD missense Z
1.8
DepMap mean gene effect
0.14
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NETO1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NETO1 as an antibody target. Whether an autoantibody or antibody against NETO1 could matter depends on whether native NETO1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NETO1 is annotated at the cell surface, where native NETO1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label NETO1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NETO1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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