Seroatlas · Human Serome Atlas

NEK4

Serine/threonine-protein kinase Nek4

Also known as: NEK4_HUMAN, NRK2, pp12301, STK2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P51957
Gene: NEK4
Ensembl: ENSG00000114904
Chromosome: 3
Canonical length: 841 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies. [provided by RefSeq, Jan 2017]

Canonical amino-acid sequenceUniProt

841 residues, UniProt reviewed canonical sequence.

>P51957|NEK4
     1  MPLAAYCYLR VVGKGSYGEV TLVKHRRDGK QYVIKKLNLR NASSRERRAA EQEAQLLSQL
    61  KHPNIVTYKE SWEGGDGLLY IVMGFCEGGD LYRKLKEQKG QLLPENQVVE WFVQIAMALQ
   121  YLHEKHILHR DLKTQNVFLT RTNIIKVGDL GIARVLENHC DMASTLIGTP YYMSPELFSN
   181  KPYNYKSDVW ALGCCVYEMA TLKHAFNAKD MNSLVYRIIE GKLPPMPRDY SPELAELIRT
   241  MLSKRPEERP SVRSILRQPY IKRQISFFLE ATKIKTSKNN IKNGDSQSKP FATVVSGEAE
   301  SNHEVIHPQP LSSEGSQTYI MGEGKCLSQE KPRASGLLKS PASLKAHTCK QDLSNTTELA
   361  TISSVNIDIL PAKGRDSVSD GFVQENQPRY LDASNELGGI CSISQVEEEM LQDNTKSSAQ
   421  PENLIPMWSS DIVTGEKNEP VKPLQPLIKE QKPKDQSLAL SPKLECSGTI LAHSNLRLLG
   481  SSDSPASASR VAGITGVCHH AQDQVAGECI IEKQGRIHPD LQPHNSGSEP SLSRQRRQKR
   541  REQTEHRGEK RQVRRDLFAF QESPPRFLPS HPIVGKVDVT STQKEAENQR RVVTGSVSSS
   601  RSSEMSSSKD RPLSARERRR LKQSQEEMSS SGPSVRKASL SVAGPGKPQE EDQPLPARRL
   661  SSDCSVTQER KQIHCLSEDE LSSSTSSTDK SDGDYGEGKG QTNEINALVQ LMTQTLKLDS
   721  KESCEDVPVA NPVSEFKLHR KYRDTLILHG KVAEEAEEIH FKELPSAIMP GSEKIRRLVE
   781  VLRTDVIRGL GVQLLEQVYD LLEEEDEFDR EVRLREHMGE KYTTYSVKAR QLKFFEENMN
   841  F

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NEK4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.51
Highest tissue expression: 43 nTPM

Expression across tissuesHPA

Tissue

testis: 43 nTPM
retina: 16 nTPM
fallopian tube: 13 nTPM
thyroid gland: 13 nTPM
parathyroid gland: 11 nTPM
ovary: 11 nTPM

Single-cell type

ependymal cells: 102 nCPM
early primary spermatocytes: 87 nCPM
late primary spermatocytes: 80 nCPM
respiratory ciliated cells: 77 nCPM
sertoli cells: 75 nCPM
fallopian tube ciliated cells: 63 nCPM

Immune cell

basophil: 13 nTPM
classical monocyte: 9.7 nTPM
intermediate monocyte: 6.1 nTPM
myeloid DC: 5.2 nTPM
total PBMC: 5.2 nTPM
eosinophil: 4.9 nTPM

Brain region

midbrain: 30 nTPM
cerebellum: 30 nTPM
choroid plexus: 29 nTPM
hypothalamus: 28 nTPM
medulla oblongata: 26 nTPM
thalamus: 26 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NEK4.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 141 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Ciliopathy

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.15
gnomAD pLI: 0
gnomAD missense Z: 0.88
DepMap mean gene effect: -0.01
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NEK4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NEK4 as an antibody target. Whether an autoantibody or antibody against NEK4 could matter depends on whether native NEK4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NEK4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NEK4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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