Seroatlas · Human Serome Atlas

NECAB2

N-terminal EF-hand calcium-binding protein 2

Also known as: EFCBP2, NECA2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q7Z6G3
Gene: NECAB2
Ensembl: ENSG00000103154
Chromosome: 16
Canonical length: 386 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

The protein encoded by this gene is a neuronal calcium-binding protein that binds to and modulates the function of at least two receptors, adenosine A(2A) receptor and metabotropic glutamate receptor type 5. [provided by RefSeq, Jul 2016]

Canonical amino-acid sequenceUniProt

386 residues, UniProt reviewed canonical sequence.

>Q7Z6G3|NECAB2
     1  MCERAARLCR AGAHRLLREP PQQGRALGGL LRWVGARMGE PRESLAPAAP ADPGPASPRG
    61  GTAVILDIFR RADKNDDGKL SLEEFQLFFA DGVLNEKELE DLFHTIDSDN TNHVDTKELC
   121  DYFVDHMGDY EDVLASLETL NHSVLKAMGY TKKVYEGGSN VDQFVTRFLL KETANQIQSL
   181  LSSVESAVEA IEEQTSQLRQ NHIKPSHSAA QTWCGSPTPA SAPNHKLMAM EQGKTLPSAT
   241  EDAKEEGLEA QISRLAELIG RLESKALWFD LQQRLSDEDG TNMHLQLVRQ EMAVCPEQLS
   301  EFLDSLRQYL RGTTGVRNCF HITAVRLSDG FTFVIYEFWE TEEAWKRHLQ SPLCKAFRHV
   361  KVDTLSQPEA LSRILVPAAW CTVGRD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NECAB2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 137 nTPM

Expression across tissuesHPA

Tissue

basal ganglia: 137 nTPM
amygdala: 126 nTPM
cerebral cortex: 97 nTPM
hypothalamus: 77 nTPM
hippocampal formation: 39 nTPM
choroid plexus: 29 nTPM

Single-cell type

late spermatids: 82 nCPM
other brain neurons: 41 nCPM
brain inhibitory neurons: 36 nCPM
oocytes: 28 nCPM
hepatocytes: 23 nCPM
neutrophils: 20 nCPM

Immune cell

neutrophil: 56 nTPM
classical monocyte: 0.8 nTPM
total PBMC: 0.1 nTPM
basophil: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM

Brain region

cerebral cortex: 160 nTPM
pons: 140 nTPM
basal ganglia: 120 nTPM
amygdala: 110 nTPM
hypothalamus: 98 nTPM
midbrain: 76 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.39
gnomAD pLI: 0
gnomAD missense Z: -4.91
DepMap mean gene effect: 0.12
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NECAB2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NECAB2 as an antibody target. Whether an autoantibody or antibody against NECAB2 could matter depends on whether native NECAB2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NECAB2 is annotated at the cell surface, where native NECAB2 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label NECAB2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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