NCALD

Protein identityUniProt · HPA

UniProt accession

P61601

Gene

NCALD

Ensembl

ENSG00000104490

Chromosome

8

Canonical length

193 aa

Protein class

Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

193 residues, UniProt reviewed canonical sequence.

>P61601|NCALD
     1  MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD
    61  ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE
   121  MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV
   181  RLLQCDPSSA GQF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NCALD can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

113 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.41

gnomAD pLI

0.89

gnomAD missense Z

1.56

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• calcium-mediated signaling
• regulation of signal transduction
• regulation of systemic arterial blood pressure
• vesicle-mediated transport

Molecular functions

• actin binding
• alpha-tubulin binding
• calcium ion binding
• clathrin binding
• tubulin binding

Cellular components

• clathrin coat of trans-Golgi network vesicle
• cytosol

Protein domainsUniProt · Pfam · InterPro

• EF-hand domain
• EF-hand domain pair
• EF-Hand 1, calcium-binding site
• Recoverin family
• EF hand domain
• EF-hand domain pair

KeywordsUniProt

• Calcium
• Lipoprotein
• Metal-binding
• Myristate
• Repeat

InteractionsUniProt · HPA

Protein binding partners of NCALD in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• GUCY2D
• DTX2
• HPCA
• HPCAL1