NANOG
Homeobox protein NANOG
Also known as: FLJ12581, FLJ40451, NANOG_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q9H9S0
- Gene
- NANOG
- Ensembl
- ENSG00000111704
- Chromosome
- 12
- Canonical length
- 305 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Canonical amino-acid sequenceUniProt
305 residues, UniProt reviewed canonical sequence.
>Q9H9S0|NANOG
1 MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL
61 LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL
121 SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS
181 YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF
241 YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM
301 QPEDVLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NANOG can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.66
- Highest tissue expression
- 0.9 nTPM
Expression across tissuesHPA
Tissue
- testis: 0.9 nTPM
- breast: 0.5 nTPM
- bone marrow: 0.4 nTPM
- lung: 0.4 nTPM
- skin: 0.3 nTPM
- liver: 0.2 nTPM
Single-cell type
- distal convoluted tubule cells: 3.3 nCPM
- foveolar cells: 2.7 nCPM
- renal connecting tubule cells: 2.3 nCPM
- renal collecting duct intercalated cells: 2.2 nCPM
- cone photoreceptor cells: 2.1 nCPM
- loop of henle epithelial cells: 2.1 nCPM
Immune cell
- plasmacytoid DC: 1.7 nTPM
- basophil: 1.4 nTPM
- neutrophil: 0.7 nTPM
- naive B-cell: 0.3 nTPM
- NK-cell: 0.3 nTPM
- classical monocyte: 0.2 nTPM
Brain region
- cerebellum: 7.5 nTPM
- white matter: 4.2 nTPM
- basal ganglia: 3.9 nTPM
- choroid plexus: 3.5 nTPM
- pons: 3.5 nTPM
- thalamus: 3.5 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.56
- gnomAD pLI
- 0.72
- gnomAD missense Z
- 0.4
- DepMap mean gene effect
- 0.18
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cell differentiation
- endodermal cell fate specification
- positive regulation of stem cell proliferation
- positive regulation of transcription by RNA polymerase II
- regulation of cell differentiation
- regulation of DNA-templated transcription
- regulation of gene expression
- regulation of transcription by RNA polymerase II
- somatic stem cell population maintenance
- stem cell population maintenance
Molecular functions
- DNA binding
- DNA-binding transcription factor activity
- DNA-binding transcription factor activity, RNA polymerase II-specific
- DNA-binding transcription repressor activity, RNA polymerase II-specific
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- RNA polymerase II transcription regulatory region sequence-specific DNA binding
- sequence-specific double-stranded DNA binding
- transcription cis-regulatory region binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of NANOG in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NANOG as an antibody target. Whether an autoantibody or antibody against NANOG could matter depends on whether native NANOG is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NANOG is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NANOG as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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