N4BP1

Protein identityUniProt · HPA

UniProt accession

O75113

Gene

N4BP1

Ensembl

ENSG00000102921

Chromosome

16

Canonical length

896 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nucleoli fibrillar center,Cytosol

OverviewNCBI Gene

Enables RNA nuclease activity; mRNA binding activity; and ubiquitin binding activity. Involved in cellular response to UV and negative regulation of viral genome replication. Predicted to be located in cytosol and nucleolus. Predicted to be active in PML body. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

896 residues, UniProt reviewed canonical sequence.

>O75113|N4BP1
     1  MAARAVLDEF TAPAEKAELL EQSRGRIEGL FGVSLAVLGA LGAEEPLPAR IWLQLCGAQE
    61  AVHSAKEYIK GICEPELEER ECYPKDMHCI FVGAESLFLK SLIQDTCADL CILDIGLLGI
   121  RGSAEAVVMA RSHIQQFVKL FENKENLPSS QKESEVKREF KQFVEAHADN YTMDLLILPT
   181  SLKKELLTLT QGEENLFETG DDEVIEMRDS QQTEFTQNAA TGLNISRDET VLQEEARNKA
   241  GTPVSELTKQ MDTVLSSSPD VLFDPINGLT PDEEALSNER ICQKRRFSDS EERHTKKQFS
   301  LENVQEGEIL HDAKTLAGNV IADLSDSSAD SENLSPDIKE TTEEMEYNIL VNFFKTMGYS
   361  QEIVEKVIKV YGPSTEPLLL LEEIEKENKR FQEDREFSAG TVYPETNKTK NKGVYSSTNE
   421  LTTDSTPKKT QAHTQQNMVE KFSQLPFKVE AKPCTSNCRI NTFRTVPIEQ KHEVWGSNQN
   481  YICNTDPETD GLSPSVASPS PKEVNFVSRG ASSHQPRVPL FPENGLHQQP EPLLPNNMKS
   541  ACEKRLGCCS SPHSKPNCST LSPPMPLPQL LPSVTDARSA GPSDHIDSSV TGVQRFRDTL
   601  KIPYKLELKN EPGRTDLKHI VIDGSNVAIT HGLKKFFSCR GIAIAVEYFW KLGNRNITVF
   661  VPQWRTRRDP NVTEQHFLTQ LQELGILSLT PARMVFGERI ASHDDRFLLH LADKTGGIIV
   721  TNDNFREFVN ESVSWREIIT KRLLQYTFVG DIFMVPDDPL GRSGPRLEEF LQKEVCLRDM
   781  QPLLSALPNV GMFDPSFRVP GTQAASTSHQ PPTRIQGAPS SHWLPQQPHF PLLPALPSLQ
   841  QNLPMPAQRS SAETNELREA LLKIFPDSEQ RLKIDQILVA HPYMKDLNAL SAMVLD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against N4BP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.49

Highest tissue expression

19 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.4

gnomAD pLI

0.62

gnomAD missense Z

1.16

DepMap mean gene effect

0.08

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to UV
• innate immune response
• negative regulation of cytokine production
• negative regulation of proteasomal ubiquitin-dependent protein catabolic process
• negative regulation of protein ubiquitination
• negative regulation of viral genome replication
• regulation of innate immune response

Molecular functions

• hydrolase activity
• mRNA binding
• RNA nuclease activity
• ubiquitin binding

Cellular components

• cytosol
• nucleolus
• nucleus
• PML body

Protein domainsUniProt · Pfam · InterPro

• Ribonuclease Zc3h12a-like, NYN domain
• ZC3H12/N4BP1 Ribonuclease and Regulator
• N4BP1, C-terminal UBA domain
• N4BP1, first type I KH-domain
• N4BP1, second type I KH-domain
• Zc3h12a-like Ribonuclease NYN domain
• N4BP1, first type I KH-domain
• N4BP1, second type I KH-domain
• N4BP1, C-terminal UBA domain
• N4BP1, UBA-like domain
• N4BP1, UBA-like domain

KeywordsUniProt

• Cytoplasm
• Hydrolase
• Immunity
• Innate immunity
• Nuclease
• Nucleus
• Phosphoprotein
• RNA-binding
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of N4BP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ITCH
• NEDD4