Seroatlas · Human Serome Atlas

MRPL20

Large ribosomal subunit protein bL20m

Also known as: FLJ10024, RM20_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9BYC9
Gene: MRPL20
Ensembl: ENSG00000242485
Chromosome: 1
Canonical length: 149 aa
Protein class: Predicted intracellular proteins, Ribosomal proteins
Subcellular location: Mitochondria

OverviewNCBI Gene

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Canonical amino-acid sequenceUniProt

149 residues, UniProt reviewed canonical sequence.

>Q9BYC9|MRPL20
     1  MVFLTAQLWL RNRVTDRYFR IQEVLKHARH FRGRKNRCYR LAVRTVIRAF VKCTKARYLK
    61  KKNMRTLWIN RITAASQEHG LKYPALIGNL VKCQVELNRK VLADLAIYEP KTFKSLAALA
   121  SRRRHEGFAA ALGDGKEPEG IFSRVVQYH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MRPL20 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.41
Highest tissue expression: 270 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 270 nTPM
heart muscle: 187 nTPM
choroid plexus: 155 nTPM
kidney: 152 nTPM
tongue: 150 nTPM
liver: 150 nTPM

Single-cell type

esophageal suprabasal cells: 523 nCPM
oocytes: 433 nCPM
hepatocytes: 430 nCPM
esophageal basal cells: 412 nCPM
esophageal apical cells: 362 nCPM
gastric progenitor cells: 351 nCPM

Immune cell

plasmacytoid DC: 290 nTPM
total PBMC: 220 nTPM
naive CD4 T-cell: 205 nTPM
naive CD8 T-cell: 200 nTPM
myeloid DC: 199 nTPM
classical monocyte: 192 nTPM

Brain region

midbrain: 107 nTPM
cerebral cortex: 104 nTPM
white matter: 99 nTPM
hypothalamus: 98 nTPM
spinal cord: 98 nTPM
choroid plexus: 95 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.82
gnomAD pLI: 0
gnomAD missense Z: -0.83
DepMap mean gene effect: -0.29
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

mitochondrial translation

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Large ribosomal subunit protein bL20
Ribosomal protein bL20, C-terminal
Ribosomal protein L20

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MRPL20 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MRPL20 as an antibody target. Whether an autoantibody or antibody against MRPL20 could matter depends on whether native MRPL20 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MRPL20 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MRPL20 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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