MGAT1

Protein identityUniProt · HPA

UniProt accession

P26572

Gene

MGAT1

Ensembl

ENSG00000131446

Chromosome

5

Canonical length

445 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

445 residues, UniProt reviewed canonical sequence.

>P26572|MGAT1
     1  MLKKQSAGLV LWGAILFVAW NALLLLFFWT RPAPGRPPSV SALDGDPASL TREVIRLAQD
    61  AEVELERQRG LLQQIGDALS SQRGRVPTAA PPAQPRVPVT PAPAVIPILV IACDRSTVRR
   121  CLDKLLHYRP SAELFPIIVS QDCGHEETAQ AIASYGSAVT HIRQPDLSSI AVPPDHRKFQ
   181  GYYKIARHYR WALGQVFRQF RFPAAVVVED DLEVAPDFFE YFRATYPLLK ADPSLWCVSA
   241  WNDNGKEQMV DASRPELLYR TDFFPGLGWL LLAELWAELE PKWPKAFWDD WMRRPEQRQG
   301  RACIRPEISR TMTFGRKGVS HGQFFDQHLK FIKLNQQFVH FTQLDLSYLQ REAYDRDFLA
   361  RVYGAPQLQV EKVRTNDRKE LGEVRVQYTG RDSFKAFAKA LGVMDDLKSG VPRAGYRGIV
   421  TFQFRGRRVH LAPPLTWEGY DPSWN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MGAT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

128 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.22

gnomAD pLI

0.99

gnomAD missense Z

1.51

DepMap mean gene effect

-0.18

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• in utero embryonic development
• protein N-linked glycosylation
• protein N-linked glycosylation via asparagine
• viral protein processing

Molecular functions

• acetylglucosaminyltransferase activity
• manganese ion binding
• alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity

Cellular components

• endoplasmic reticulum-Golgi intermediate compartment membrane
• extracellular exosome
• extracellular vesicle
• Golgi apparatus
• Golgi membrane
• membrane
• perinuclear region of cytoplasm

Protein domainsUniProt · Pfam · InterPro

• Glycosyl transferase, family 13
• Nucleotide-diphospho-sugar transferases
• GNT-I family
• Glycosyltransferase 13

KeywordsUniProt

• Cytoplasm
• Disulfide bond
• Glycosyltransferase
• Golgi apparatus
• Manganese
• Membrane
• Metal-binding
• Signal-anchor
• Transferase
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of MGAT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• BRI3
• MGAT4D